BsaI Restriction Endonuclease Featured Image

Endonuclease ea Thibelo ea BsaI

Bohloeki: 20U/μL

Pakete: 50μL, 250μL, 1ml, 10ml, 100ml

Lintlha tsa Sehlahisoa

Tlhaloso

Enzyme e thibelang BsaI ke mofuta oa II restriction endonuclease e nkiloeng ho recombinant protein encoded ke mofuta oa BspQI ho Bacillus sphaericus e hlahisoang ke E.coli.Li-endonuclease tse thibelang li sebelisoa haholo ho 'mapa oa liphatsa tsa lefutso, cloning le masimong a mang.Enzyme ena e potlakisa ho fokotsa DNA bakeng sa ho etsa liphatsa tsa lefutso tse sebetsang hantle Bsa I , IIs restriction endonuclease restriction endonuclease, e nkiloeng ho mofuta oa recombinant E. coli o jereng mofuta oa BsaI o hlophisitsoeng le o fetotsoeng ho tsoa ho Bacillus stearothermophilus.tse nang le ts'ebetso e ts'oanang le ea li-enzyme tsa tlhaho le ts'ebetso e fokotsehileng ea linaleli.Tatelano ea eona e tsebahalang le liwebsaete tsa cleavage ke tse latelang:

5'······GGTC(N)·········3'

3'······CCAGAG(NNNNN)······5'

Sebopeho sa Lik'hemik'hale

Likarolo tsa sehlahisoa

• Thibelo ea endonuclease e na le ts'ebetso e phahameng ea enzymatic mme e phethela karabelo ea tšilo ea lijo kapele.

• Ts'ebetso e tlase e sa tobang ea endonuclease ho netefatsa ho itšeha "scalpel" ka nepo.

• Ha ho tsamaiso ea BSA, e fokotsang tšilafalo ea mohloli oa mocheso.

Tlhaloso

Lintho tsa Tlhahlobo	Litlhaloso
Ts'ebetso ea enzyme	20 U/µL
Bohloeki(SDS-PAGE)	≥95%
Rnase	Ha ho e fumanoeng
Ts'ebetso e sa Totobalang ea DNase	Ha e fumanwe
Naleli Mosebetsi	Ha e fumanwe
Ligation le Recutting	Ka mor'a tšilo ea lijo, likaroloana tsa DNA li ka kopanngoa.'Me likhechana tsena tsa ligated li ka khaoloa hape ka Bsal.
Endotoxins(tlhahlobo ea LAL)	≤10 EU/mg
E.Coli DNA	≤1 Khopi/2U

Taolo ea boleng

Bohloeki ≥ 95%, Ha ho DNase, mosebetsi oa RNase, masala a Host DNA ≤ 100pg/mg, masala a protheine a amohelang ≤ -50ppm, masala a endotoxin ≤ 10EU/mg, Ha ho mosebetsi oa protease, nyopa, o se nang mycoplasma

Kopo

Cheka plasmid ho lokisa sekhechana sa DNA se nang le mela qetellong ea Poly (A/T/C/G);

Hlahisa template ea plasmid pele ho mongolo oa in vitro;

Thibelo digest;

Tlhaloso ea Yuniti

Yuniti e le 'ngoe e hlalosoa e le palo ea enzyme e hlokahalang ho sila 1 µg ea DNA ea taolo ea ka hare ka hora e le 1 ho 37 ° C ka kakaretso ea karabelo ea 50 µL.