Recombinant Trypsin
Molemo
1.Ha ho tlhaho ea liphoofolo
2.Bohloeki bo phahameng
3.Better mokhoa oa ho laola
4.Boleng bo tsitsitseng
Tlhaloso
Trypsin ka ho khetheha e khaola libopo tsa C-terminal peptide tsa lysine le arginine, tse ka senyang liprotheine tse tlamang ka har'a lisele.Tatelano ea amino acid ea recombinant trypsin e hlahisoang ke Hzymes Biotech e tšoana le ea porcine pancreas-derived trypsin, 'me e hlahisoa ke recombinant Escherichia coli expression.E ka nkela sebaka sa setso sa trypsin bakeng sa tšilo ea lisele ka liente, lisele tsa stem, kalafo ea lisele tsa 'mele, tlhahlobo ea lithethefatsi, li-antibodies le likarolo tse ling.Aprotinin, soybean trypsin inhibitor, joalo-joalo e ka thibela ts'ebetso ea trypsin ka mokhoa o hlakileng.
Recombinant trypsin e na le thepa e tšoanang le ea trypsin e nkiloeng ka tlhaho.Sehlahisoa sena se entsoe ka har'a workshop ea boemo ba GMP.Ha ho lisebelisoa tse tala tsa liphoofolo tse sebelisoang ho belisoa, ho hloekisa le metsoako ea ho qetela.Ka nako e ts'oanang, mekhoa ea ho hlahisa le ho laola boleng e tiisitsoe, e leng se etsang hore ho be le boemo bo phahameng ba ho lumellana pakeng tsa lihlopha.
Sebopeho sa Lik'hemik'hale
Tlhaloso ea Yuniti
25 ℃, pH 7.6, 3.2ml reaction solution(1cm light path), yuniti e le 'ngoe ea trypsin (U) e ile ea hlalosoa e le
keketseho ea 0.003 boleng ba ho monya ho 253nm ka enzymatic hydrolysis ea BAEE ka motsotso.
Tšebeliso ea Sehlahisoa
Bakeng sa setso sa sele
• Tšimoloho ea lithane tse thibelang lijo, ho fumana lisele tsa mantlha.
• Tšilo ea lijo ea lisele tse khomarelang.
• Setso sa lisele ka mokhoa oa microcarrier.
• Ho sila lisele tsa stem ka bonolo.
• Kalafo ea lisele tsa 'mele, joalo-joalo.
Bakeng sa recombinant protheine
• Tlhahiso ea insulin e kopanetsoeng.
• Tatellano ea liprotheine, 'mapa oa peptide.
• Mekhoa e ikhethileng ea proteolytic joalo ka lipatlisiso tsa proteomics
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Ponahalo | Phofo e tšoeu kapa e sa koaeloang |
| Sollubility (10mg/ml) | Tharollo e hlakileng e se nang mebala |
| Bohloeki | Β-trypsin ≥70%, α-trypsin ≤ 20% |
| Ts'ebetso e khethehileng (Phofo) | ≥2500U/mg |
| E.Coli e amohela liprotheine tse setseng | ≤0.01% |
| Teko ea Endotoxins(LAL-Test) | ≤20 EU/mg |
Lipalangoang le polokelo
Lipalangoang:E romelloa ka tlas'a 2-8 °C
Polokelo:Boloka ho -2-8 ° C, ka mor'a hore e qhibilihe ka 1mM hcl kapa 50nM HAC, e lokela ho bolokoa ka tlas'a -25 ~ -15 °C
E khothalelitsoe ho lekoa hape Bophelo:2 lilemo
