Deoxyribonuclease I(Dnase I)

DNase I (Deoxyribonuclease I) ke endodeoxyribonuclease e ka silang DNA e nang le mela e le 'ngoe kapa e habeli.E amohela le ho qhetsola libond tsa phosphodiester ho hlahisa monodeoxynucleotides kapa oligodeoxynucleotide e nang le stranded e le 'ngoe kapa habeli ka lihlopha tsa phosphate sebakeng sa 5'-terminal le hydroxyl ho 3'-terminal.Mosebetsi oa DNase I o itšetlehile ka Ca 2+ 'me o ka sebelisoa ke li-ion tsa tšepe tse fapaneng tse kang Mn 2+ le Zn 2+.5 mM Ca 2+ e sireletsa enzyme ho tsoa ho hydrolysis.Ha Mg 2+ e le teng, enzyme e ne e khona ho lemoha le ho petsola sebaka leha e le sefe sa DNA.Ka pel'a Mn 2+, likhoele tse peli tsa DNA li ka hlokomeloa ka nako e le 'ngoe' me tsa phunyeletsoa hoo e batlang e le sebakeng se le seng ho etsa likaroloana tsa DNA kapa likhechana tsa DNA tse nang le li-nucleotide tse 1-2 tse hlaheletseng.