Bst 2.0 DNA Polymerase (Glycerol mahala)
Bst DNA polymerase V2 e tsoa ho Bacillus stearothermophilus DNA Polymerase I, e nang le ts'ebetso ea 5′→3' DNA polymerase le ts'ebetso e matla ea ketane, empa ha ho na 5′→3′ exonuclease.Bst DNA Polymerase V2 e loketse hantle bakeng sa ho falla ha strand, isothermal amplification LAMP (Loop mediated isothermal amplification) le tatellano e potlakileng.
Likaroloana
| Karolo | HC5005A-01 | HC5005A-02 | HC5005A-03 |
| BstDNApolymerase V2(Glycerol-mahala)(8U/μL) | 0.2 mL | 1 mL | 10 ml |
| 10 × HC Bst V2 Buffer | 1.5 mL | 2×1.5 mL | 3 × 10 mL |
| MgSO4(100mM) | 1.5 mL | 2×1.5 mL | 2 × 10 mL |
Lisebelisoa
1.LAMP isothermal amplification
2.DNA strand single displacement reaction
3.High GC gene tatelano
4.Tlhaloso ea DNA ea boemo ba nanogram.
Boemo ba polokelo
Lipalangoang ka tlas'a 0°C 'me li bolokoe ho -25°C~-15°C.
Tlhaloso ea Yuniti
Yuniti e le 'ngoe e hlalosoa e le palo ea enzyme e kenyelletsang 25 nmol ea dNTP linthong tse sa keneng tsa asiti ka metsotso e 30 ho 65 ° C.
Taolo ea Boleng
1.Protein Purity Assay (SDS-PAGE):Bohloeki ba Bst DNA polymerase V2 ke ≥99% e khethiloeng ke tlhahlobo ea SDS-PAGE ho sebelisoa Coomassie Blue discovery.
2.Ts'ebetso ea Exonuclease:Ho kenyeletswa ha 50 μL reaction e nang le bonyane ba 8 U of Bst DNA polymerase V2 e nang le 1 μg λ -Hind Ⅲ digest DNA bakeng sa dihora tse 16 ka 37 ℃ e fella ka hore ho se be le tshenyeho e lemohuwang jwalo ka ha ho laetswe.
3.Mosebetsi oa Nickase:Ho kenyeletswa ha 50 μL karabelo e nang le bonyane ba 8 U of Bst DNA polymerase V2 e nang le 1 μg pBR322 DNA bakeng sa dihora tse 16 ka 37°C e fella ka hore ho se be le tshenyeho e lemohuwang jwalo ka ha ho hlalositswe.
4.Mosebetsi oa RNase:Ho kenyeletswa ha 50 μL karabelo e nang le bonyane ba 8 U of Bst DNA polymerase V2 e nang le 1.6 μg MS2 RNA bakeng sa dihora tse 16 ka 37°C e fella ka hore ho se be le tshenyeho e lemohuwang jwalo ka ha ho hlalositswe.
5.E. coli DNA:120 U of Bst DNA polymerase V2 e hlahlojoa boteng ba E. coli genomic DNA e sebelisa TaqMan qPCR e nang le li-primers tse tobileng bakeng sa sebaka sa E. coli 16S rRNA.Tšilafalo ea E. coli genomic DNA ke ≤1 Kopi.
LEMPA tsiboso
| Likaroloana | 25μL |
| 10 × HC Bst V2 Buffer | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTPs (10mM e 'ngoe le e 'ngoe) | 3.5 μL |
| SYTO™ 16 e tala (25×)a | 1.0 μL |
| Motsoako oa peleb | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-mahala) (8 U/uL) | 1 μL |
| Setšoantšo | × μL |
| ddH₂O | Ho fihlela ho 25 μL |
Lintlha:
1) a.SYTOTM 16 Green (25 ×): Ho ea ka litlhoko tsa liteko, li-dyes tse ling li ka sebelisoa e le li-substitutes;
2) b.Primer mix: e fumanoe ka ho kopanya 20 µ M FIP, 20 µ M BIP, 2.5 µ M F3, 2.5 µ M B3, 5 µ M LF, 5 µ M LB le mefuta e meng.
Boitšoaro le Boemo
1 × HC Bst V2 Buffer, mocheso oa incubation o pakeng tsa 60°C le 65°C.
Ho se sebetse ha mocheso
80 ° C, metsotso e 20
