Multiplex One Step RT-qPCR Probe Kit (UDG Plus)
Tlhaloso
Multiplex One Step RT-qPCR Kit (UDG Plus) ke sesebelisoa sa ho lemoha ka potlako sa RT-qPCR se loketseng single-plex kapa multi-plex quantitative PCR se sebelisang RNA joalo ka template (joalo ka vaerase ea RNA).E sebelisoa ho holisa DNA e habeli ho tsoa ho litempele tsa RNA tse nang le metsu e le 'ngoe ho lumella palo e potlakileng ea nako ea nnete ea lipehelo tsa RNA.Mothating oa ho ngola ka morao, reverse transcriptase e kopanya limolek'hule tsa DNA tse nang le mela e le 'ngoe (cDNA) e tlatselletsang template ea RNA.
Mokhoa oa ho itšoara
Likaroloana
| Likaroloana | 200rxns | 1,000rns | 10,000 rxns |
| RNase-free ddH2O | 3*1ml | 15ml | 150ml |
| 5* mohato o le mong motsoako | 800μl | 4*1ml | 40ml |
| Motsoako o le mong oa enzyme | 200μl | 1ml | 10ml |
| 50* ROX reference Dye 1 | 80μl | 400μl | 4*1ml |
| 50* ROX reference Dye 2 | 80μl | 400μl | 4*1ml |
a.Mohato o le Mong Buffer e kenyelletsa dNTP Mix le Mg2+.
b.Enzyme Mix e na le reverse transcriptase, Hot Start Taq DNA polymerase (phetoho ea antibody) le RNase inhibitor.
c.E sebelisoa ho lokisa phoso ea lithapo tsa fluorescene lipakeng tsa liliba tse fapaneng.
c.ROX: U hloka ho khetha calibration ho latela mohlala oa sesebelisoa sa tlhahlobo.
Lisebelisoa
Ho fumanoa ha likokoana-hloko
tlhahlobo le lipatlisiso tsa hlahala
Ho lemoha mafu a liphoofolo
Litlhahlobo tsa pele tsa mafu a lefutso
Ho fumanoa ha likokoana-hloko tsa pathogenic lijong
Thomello le Polokelo
Lipalangoang:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -30 ~ -15 ℃.
Bophelo ba Shief:1 lilemo
