Recombinant Deoxyribonuclease I (DNase I, RNase-free)
Tlhaloso
Recombinant Deoxyribonuclease I, ke endonuclease e khonang ho sila DNA e nang le thipa e le 'ngoe kapa e nang le likarolo tse peli.E hydrolyzes phosphodiester litlamo ho hlahisa mono- le oligodeoxynucleotides tse nang le lihlopha tsa 5'-phosphate le lihlopha tsa 3'-OH.Mokhoa o nepahetseng oa ho sebetsa oa pH oa DNase I ke 7-8.Mosebetsi oa DNase I o itšetlehile ka Ca2 + 'me o ka sebelisoa ke li-ion tsa tšepe tsa divalent tse kang Co2+, Mn2+, Zn2+, joalo-joalo Ka pel'a Mg2 +, DNase Ke khona ho khaola DNA e nang le likarolo tse peli sebakeng leha e le sefe;ha ka pel'a Mn2+, DNase ke khona ho khaola DNA ka makhetlo a mabeli sebakeng se le seng, ho etsa lipheletsong tse lerootho kapa li-nuclei tse 1-2 tse Sticky tse nang le li-nucleotide overhangs.Enzyme ena e nkiloe mefuteng e kopaneng ea E. coli, ha e na RNase, 'me e ka sebelisoa bakeng sa ho lokisa lisampole tse fapaneng tsa RNA.
Sebopeho sa lik'hemik'hale
Lisebelisoa
RNA extract: lokisetsa RNA ntle le DNA;
Kamora ho ngoloa ka in vitro ka RNA polymerase, joalo ka T7 RNA Polymerase (Cat#10618), e sebelisoa ho tlosa template ea DNA;
Lokisetsa RNA e se nang DNA pele ho RT-PCR le RT-qPCR;
E sebelisoa hammoho le DNA Polymerase I (Cat#12903) bakeng sa ho ngola DNA ka phetolelo ea bo-nick;
E sebelisoa tlhahlobong ea maoto ho sekaseka tšebelisano ea protheine ea DNA;
Hlahisa laeborari ea likhechana tse sa reroang;
Ho fumanoeng ka TUNEL ea apoptosis, DNA ea genomic e sehiloeng ka mokhoa o itseng e sebelisitsoe e le taolo e ntle.
Thomello le Polokelo
Lipalangoang:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -20 ℃
Shelf Life:lilemo tse 2
Lintlha:
1.Li-enzyme li lokela ho bolokoa ka lebokoseng la leqhoa kapa ka holim'a leqhoa ha li sebelisoa, 'me li lokela ho bolokoa ho -20 ° C hang ka mor'a ho sebelisoa.
2. Bakeng sa polokeho le bophelo bo botle ba hau, ka kopo apara lisebelisoa tsa ts'ireletso (PPE), joalo ka lijase tsa laboratori le liatlana tse lahloang, ha u sebetsa ka sehlahisoa sena.
