RT-LAMP Reagent (-20℃)
Tlhaloso
LAMP hajoale e sebelisoa haholo thekenoloji lefapheng la amplification ea isothermal.E sebelisa li-primers tsa 4-6 tse ka khethollang libaka tse 6 tse khethehileng ho mofuta o lebisitsoeng, 'me e itšetlehile ka ts'ebetso e matla ea ho falla ea Bst DNA polymerase.Ho na le mekhoa e mengata ea ho lemoha LAMP, ho kenyelletsa le mokhoa oa ho daea, mokhoa oa pH colorimetric, mokhoa oa turbidity, HNB, calcein, joalo-joalo RT-LAMP ke mofuta o mong oa karabelo ea LAMP e nang le RNA e le template.RT-LAMP Kit (UDG plus) e thehiloe ho latela mokhoa oa fluorescence 'me e na le Bst DNA Polymerase, thermotolerant reverse transcriptase, murine RNase inhibitor, le UDGase.Sesebelisoa sena sa LAMP se na le kutlo e phahameng, ts'ebeliso e ntle ea primer, le ts'ebetso e ntle haholo ka ho khetheha, mamello le botsitso.
Likaroloana
| 100T | 1000T |
| 600μL | 6mL |
| 100 μL | 1 mL |
1) Bst Buffer e na le dN(U)TP le SYTO-9, joalo-joalo, le 1×Bst Buffer e se e na le 8 mmol/L Mg2+;
2) Bst Enzyme Mix (UDG plus) e na le UDGase, Reverse Transcriptase, Bst Ⅱ Polymerase, MRI;
Lisebelisoa
Liketso tsa LAMP
Thomello le Polokelo
Lipalangoang:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -20 ℃
Bophelo ba Shief:Likhoeli tse 6
Litemoso
Bakeng sa polokeho le bophelo bo botle ba hau, ka kopo roala lijase tsa lab le liatlana tse lahloang bakeng sa ts'ebetso.
