T4 RNA ligase
Tlhaloso
Sehlahisoa sena ke T4 RNA ligase e itšetlehileng ka ATP (T4 RNA Ligase I) e hlahisoang hape ke E. coli.Enzyme ena e etsa hore ho thehoe maqhama a phosphodiester pakeng tsa oligonucleotides, RNA e le 'ngoe le DNA intermolecular / intra-molecular 5'-PO4 le 3'-OH.
Tlhaloso ea Yuniti
Yuniti e le 'ngoe e hlalosoa e le palo ea enzyme e hlokahalang ho fetola nanomole e le 1 ea 5'-[³²P] rA16 hore e be sebopeho se hananang le phosphatase ka metsotso e 30 ho 37°C.
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Ts'ebetso ea enzyme | 10 U/μL |
| Ts'ebetso ea protheine | ≥ 95% |
| Nickase | Ha e bonahale |
| Mosebetsi oa RNase | Ha e bonahale |
| Ecoli gDNA | ≤1kopi/50U |
| Endotoxin | LCL-Test,≤10EU/mg |
Lipalangoang le polokelo
Lipalangoang:Leqhoa le omileng
Polokelo:E boloke ho -25 ~ 15 ° C (qoba ho haama khafetsa le ho qhibiliha)
E khothalelitsoe ho lekoa hape Bophelo:2 lilemo
lihlahisoa tse amanang
Ngola molaetsa wa hao mona mme o re romele wona
