Endonuclease ea Thibelo ea BspQI
Tlhaloso
BspQI e ka hlahiswa hape ka E. coli e ka lemohang dibaka tse itseng mme e hlahiswa tlasa maemo a GMP.E sebelisoa haholo ho 'mapa oa liphatsa tsa lefutso, cloning le masimong a mang.Enzyme ena e poma DNA ka potlako bakeng sa ho tsamaisa liphatsa tsa lefutso hantle.
5'······GGTC(N)1······3'
3'······CCAGAG(N)4······5'
Sebopeho sa Lik'hemik'hale
Tlhaloso ea Yuniti
Yuniti e le 'ngoe e hlalosoa e le palo ea enzyme e hlokahalang ho cheka 1 µg ea λ DNA ka hora e le 1 ho 50 ° C ka kakaretso ea karabelo ea 50 µl.
Likarolo tsa Sehlahisoa
• Thibelo ea endonuclease e na le ts'ebetso e phahameng ea enzymatic mme e phethela tšilo ea lijo kapele.
• Ts'ebetso e tlase e sa tobang ea endonuclease ho netefatsa ho itšeha "scalpel" ka nepo.
• Ha ho tsamaiso ea BSA, e fokotsang tšilafalo ea mohloli oa mocheso.
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Bohloeki ba Protheine | 98% |
| Masala a protease | Ha ho e fumanoeng |
| Khokahano le ho fumana sehiloeng | Ho sebetsa hantle ha tšilo ea lijo ~95% |
| DNase | Ha ho e fumanoeng |
| Nicaknase | Ha ho e fumanoeng |
Lipalangoang le polokelo
Lipalangoang:E rometsoe ka tlas'a 0 °C
Polokelo:Boloka ho -20 °C
E khothalelitsoe ho lekoa hape Bophelo:2years
