DNase assay kit (Fluorescence)
Tlhaloso
Thepa ea ho lemoha ea DNase e thehiloe holim'a tlhahlobo ea DNA e ngotsoeng ke fluorophore.Ha sampuli e se na mosebetsi oa DNase, probe e tsitsitse ebile ha e hlahise letšoao la fluorescent;ha sampole e na le ts'ebetso ea DNase, probe ea senyeha, e bakang lets'oao la fluorescence le ntlafalitsoeng butle;tekanyo ea ho eketseha ha pontšo ea fluorescence e amana hantle le palo le mosebetsi oa li-enzyme.Sebelisa sebali sa microplate sa fluorescence ho lekanya ho wavelength ea ex/em=485/525nm ho fumana hore na sampole e silafalitsoe ke DNase
Sebopeho sa Lik'hemik'hale
Sebopeho sa Kit
| Lebitso | 48Tlhahlobo | 192Tlhahlobo |
| 10 × tharollo ea karabelo | 0.5mL | 2.0mL |
| DNA lipatlisiso | 1 tube | 1 tube |
| TE buffer | 0.5mL | 2.0mL |
| DNase I e tloaelehileng (2U/μL) | 10μL | 20μL |
| Tloaelehileng Dilution Buffer | 6mL | 12mL |
| DNase (metsi a se nang RNase). | 25mL | 25mL |
| DNase RNase hole | 50mL | 50mL |
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Ponahalo | Ho phuthela ho feletseng 'me ha ho na metsi a lutlang |
| Moeli oa ho lemoha | DNase I: 1.25x10-6U/μL |
| Nepahalo | Intra assay CV ≤ 10% |
Lipalangoang le polokelo
Lipalangoang:Leqhoa le omileng
Polokelo:E boloke ho -25 ~ 15 ° C (qoba ho haama khafetsa le ho qhibiliha)
E khothalelitsoe ho lekoa hape Bophelo:1 selemo
lihlahisoa tse amanang
Ngola molaetsa wa hao mona mme o re romele wona
