RNase assay kit (Fluorescence)
Tlhaloso
Thepa ea ho lemoha ea RNase e thehiloe holim'a tlhahlobo ea RNA e ngotsoeng ka fluorophore, e thusang bafuputsi ho lekanya tšebetso ea RNase.Ha sampuli e se na mosebetsi oa RNase, probe e tsitsitse ebile ha e hlahise letšoao la fluorescent;ha sampole e na le ts'ebetso ea RNase, probe ea senyeha, e bakang lets'oao la fluorescence le ntlafalitsoeng butle;Sekhahla sa keketseho ea lets'oao la fluorescence se amana hantle le palo le ts'ebetso ea li-enzyme.
Sebopeho sa Lik'hemik'hale
Sebopeho sa Kit
| Lebitso | 48Tlhahlobo | 192Tlhahlobo |
| 10 × tharollo ea karabelo | 0.5mL | 2.0mL |
| Setšoantšo sa RNA | 1 tube | 1 tube |
| TE buffer | 0.5mL | 2.0mL |
| RNase A e tloaelehileng (10mg/mL) | 10μL | 20μL |
| Tloaelehileng Dilution Buffer | 6mL | 12mL |
| DNase (metsi a se nang RNase). | 25mL | 25mL |
| DNase RNase hole | 50mL | 50mL |
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Ponahalo | Ho phuthela ho feletseng 'me ha ho na metsi a lutlang |
| Moeli oa ho lemoha | RNase A: 0.313pg/mL |
| Nepahalo | Intra assay CV ≤ 10% |
Lipalangoang le polokelo
Lipalangoang:Leqhoa le omileng
Polokelo:Boloka ho -20°C (qoba serame khafetsa le ho qhibiliha)
E khothalelitsoe ho lekoa hape Bophelo:1 selemo
lihlahisoa tse amanang
Ngola molaetsa wa hao mona mme o re romele wona
