RT-LAMP Fluorescent (bolo ea Lyophilized)
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) e na le "reaction buffer", Bst DNA polymerase le reverse transcriptase e thibelang mocheso, e sireletsang lyophilized, le dae ea fluorescent.Sehlahisoa se ka sebopeho sa lifaha tse nang le lyophilized, 'me ke li-primers le li-template feela tse lokelang ho eketsoa bakeng sa tšebeliso.
Karolo ea Reagent
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Tšebeliso
Bakeng sa DNA kapa RNA isothermal amplification
Maemo a polokelo
Lipalangoang le ho boloka ho 2~8°C.
Shelf bophelo: 12 likhoeli.
Protocol
1. Ntša palo e tsamaellanang ea lifaha tsa lyophilized ho latela palo ea liteko.
2. Ho lokisetsa mokhoa oa ho itšoara
| Karolo | Tekanyetso |
| RT-LAMP Fluorescent Master Mix (Phofo ea Lyophilized) | 2 lifaha |
| Dae ea fluorescent | x μL |
| 10 × Primer Mix a | 5 μL |
| Lithempleite DNA/ RNA b | n μL |
| Kakaretso | 50 μL |
Lintlha:
1) a.10 × Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) b.Thempleite ea nucleic acid e khothalletsoa hore e qhibilihe ka metsing a DEPC.
Hyasen Biotechnology Co., Ltd
3) Lenaneo la ho itšoara
| Mocheso | Nako | Lipotoloho |
| 65℃ | 60s a | 30 |
Lintlha:
1. Bokella letšoao la fluorescent
Lintlha
1. Thempereichara ea karabelo e ka ntlafatsoa lipakeng tsa 62°C le 68°C ho latela primer.maemo;
2. Teko e lokela ho tiisa ts'ebetso, ho kenyelletsa le ho lokisoa ha sistimi ea karabelo,ts'ebetso ea sampole le dosing;
3. Ho qoba tšilafalo, ho kgothaletswa ho lokisetsa mokhoa oa ho itšoara ka tafoleng e hloekileng haholo'me u kenye litempele ho hood ea mosi ka likamoreng tse ling ho qoba tšitiso e fosahetseng.
