ELEISA KIT bakeng sa Trypsin
Tlhaloso
Recombinant Trypsin e sebelisoa khafetsa tlhahisong ea biopharmaceutical-nakong ea ho lokisoa ha lisele kapa ho fetola le ho kenya tšebetsong lihlahisoa.Trypsin e baka likotsi tsa polokeho, ka hona e tlameha ho tlosoa pele sehlahisoa sa ho qetela se lokolloa.Sandwich Kit ena ke ea tekanyo ea tekanyo ea Residual Trypsin ho cell culture supernatant le mekhoa e meng ea tlhahiso ea biopharmaceutical ha Trypsin e sebelisoa.
Sekhahla sena ke Tlhahlobo ea Immunosorbent ea Enzyme-Linked (ELISA).Letlapa le kentsoe pele ka lesole la 'mele la Porcine trypsin.Trypsin e teng sampoleng ea eketsoa 'me e tlama ho li-antibodies tse koahetsoeng ka lilibeng.Ebe biotinylated Porcine trypsin antibody ea eketsoa ebe e tlama ho trypsin sampoleng.Ka mor'a ho hlatsoa, HRP-Streptavidin e kenngoa 'me e tlama ho antibody ea trypsin ea Biotinylated.Ka mor'a ho qhoqhoa, HRP-Streptavidin e hlatsuoa.Ebe tharollo ea TMB substrate e ea eketsoa le ho hlophisoa ke HRP ho hlahisa sehlahisoa sa 'mala o moputsoa se fetohileng mosehla kamora ho eketsa tharollo ea acidic stop.Boima ba 'mala o mosehla bo lekana le tekanyo ea trypsin
sampole e hapiloeng ka poleiti.Monyetla o lekanngoa ho 450 nm.
Sebopeho sa Lik'hemik'hale
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Ponahalo | Ho phuthela ho feletseng 'me ha ho na metsi a lutlang |
| Moeli o tlase oa ho lemoha | 0.003 ng/mL |
| Moeli o tlase oa bongata | 0.039 ng/mL |
| Nepahalo | Intra assay CV≤10% |
Lipalangoang le polokelo
Lipalangoang:Ambient
Polokelo:E ka bolokoa ho -25 ~ -15 ° C bophelong ba sethala, 2-8 ° C bakeng sa boiketlo bo bong ba liteko.
E khothalelitsoe ho lekoa hape Bophelo:1 selemo
