Alkaline Phosphatase(ALP)

Cas No.: 9001-78-9

Bohloeki: 90%

Pakete: 100μL, 500μL, 10mL, 100mL, 1000mL

Lintlha tsa Sehlahisoa

Tlhaloso

Alkaline Phosphatase e tsoa mofuteng oa motsoako oa E. coli o jereng lefutso la TAB5.Enzyme e susumetsa dephosphorylation ea 5' le 3' lipheletsong tsa DNA le RNA phosphomonoesters.Hape, e etsa hydrolyses ribose, hammoho le deoxyribonucleoside triphosphates (NTPs le dNTPs).TAB5 Alkaline Phosphatase e sebetsa ho 5' ho hlahella, 5' ho khutlela morao le lipheletsong tse lerootho.Phosphatase e ka sebelisoa lits'ebetsong tse ngata tsa baeloji ea limolek'hule, joalo ka cloning kapa probe end label ho tlosa lipheletso tsa phosphorylated tsa DNA kapa RNA.Litekong tsa cloning, dephosphorylation e thibela DNA ea plasmid ea linearized hore e se ke ea itšehla thajana.E ka boela ea theola li-dNTP tse sa kenyelletsoeng karabelong ea PCR ho lokisa template bakeng sa tatellano ea DNA.Enzyme ha e sebetse ka botlalo ebile e ke ke ea fetoloa ka ho futhumatsa ho 70 ° C metsotso e 5, ka hona, ho tlosa phosphatase pele ho ligation kapa ho emisa ho ngola ho sa hlokahale.

Sebopeho sa Lik'hemik'hale

Tlhaloso

Lintho tsa Tlhahlobo	Litlhaloso
Ts'ebetso ea enzyme	5U/μL
Bohloeki	≥ 95%
Ts'ebetso ea Endonuclease	Ha e bonahale
Ts'ebetso ea Exonuclease	Ha e bonahale
Ts'ebetso ea Nicking	Ha e bonahale
Mosebetsi oa RNase	Ha e bonahale
E.coli DNA	≤1kopi/5U
Endotoxin	Tlhahlobo ea LAL, ≤ 10EU/mg