Ultra Nuclease

Cas No.: 9025-65-4

EC.No.: 3.1.30.2

Pakete: 20μL, 200μL, 2mL, 20mL

Lintlha tsa Sehlahisoa

Tlhaloso

UltraNuclease ke endonuclaese e entsoeng ka liphatsa tsa lefutso e nkiloeng ho Serratia marcescens, e khonang ho theola DNA kapa RNA, ebang ke habeli kapa e le 'ngoe, e pota-potiloe kapa e chitja tlas'a maemo a mangata, e senya ka ho feletseng li-nucleic acid ho 5'-monophosphate oligonucleotides ka 3-5. bolelele ba motheo.

Ka mor'a phetoho ea liphatsa tsa lefutso, sehlahisoa se ile sa belisoa, sa hlalosoa, 'me sa hloekisoa ho Escherichia coli (E. coli), e fokotsang viscosity ea cell supernatant le cell lysate lipatlisisong tsa saense, empa hape e ntlafatsa ts'ebetso ea tlhoekiso le lipatlisiso tse sebetsang tsa protheine.E ka boela ea sebelisoa indastering ea meriana ea liphatsa tsa lefutso, ho hloekisa kokoana-hloko, tlhahiso ea ente, liprotheine le polysaccharide e le setsi sa ho tlosa masala a nucleic acid.

Sebopeho sa Lik'hemik'hale

Tlhaloso ea Yuniti

Palo ea enzyme e sebelisetsoang ho fetola boleng ba ho monya △A260 ka 1.0 ka hare ho 30min ho 37 °C, pH 8.0, e lekanang le 37μg ea salmon ea peō ea DNA e entsoeng ka oligonucleotides, e hlalositsoe e le karolo e sebetsang.

Tšebeliso le Tekanyetso

• Tlosa exogenous nucleic acid lihlahisoa tsa ente, fokotsa kotsi ea masala a chefo ea nucleic acid le ho ntlafatsa polokeho ea sehlahisoa.
• Fokotsa viscosity ea mokelikeli oa phepelo o bakoang ke nucleic acid, khutsufatsa nako ea ho sebetsa le ho eketsa chai ea protheine.
• Tlosa nucleic acid e neng e phuthetsoe ka karoloana (vaerase, 'mele oa ho kenyelletsa, joalo-joalo), e leng se loketseng ho lokolloa le ho hloekisa karoloana.
• Kalafo ea nyutlelie e ka ntlafatsa tharollo le ho hlaphoheloa ha sampuli bakeng sa kholomo ea chromatography, electrophoresis le tlhahlobo ea blotting.
• Ka phekolo ea liphatsa tsa lefutso, nucleic acid e tlosoa ho fumana likokoana-hloko tse amanang le adeno tse hloekisitsoeng.

Tlhaloso

Lintho tsa Tlhahlobo	Litlhaloso
Tlhaloso	E hlakileng le e se nang 'mala
Ketsahalo	≥ 250 U/ul
Ts'ebetso e khethehileng	≥1.1*10⁶U/mg
Bohloeki (SDS-PAGE)	≥ 99.0%
Maprotease	Ha ho e fumanoeng
Bioburden	＜10 cfu/100,000U
Endotoxines(tlhahlobo ea LAL)	＜ 0.25EU/1,000U