Ribonuclease A (RNase A)

Ribonuclease A (RNase A) ke polypeptide e nang le khoele e le 'ngoe e nang le li-bond tsa 4 tsa disulfide tse nang le boima ba molek'hule bo ka bang 13.7 kDa.RNase A ke endoribonuclease e nyenyefatsang ka ho khetheha RNA ea makhalo a le mong ho masala a C le U.Haholo-holo, cleavage e lemoha tlamo ea phosphodiester e entsoeng ke 5'-ribose ea nucleotide le sehlopha sa phosphate holim'a 3'-ribose ea pyrimidine nucleotide e haufi, e le hore 2', 3' - Cyclic phosphates e hydrolyzed ka tsela e tšoanang. 3'-nucleoside phosphates (mohlala, pG-pG-pC-pA-pG e petsotsoe ke RNase A ho hlahisa pG-pG-pCp le A-PG).RNase A ke eona e sebetsang ka ho fetesisa ho petsola RNA ea makhalo a le mong, 'me e sebetsa maemong a fapaneng a karabelo: maemong a tlase a letsoai (0 ho isa ho 100 mM NaCl), e ka sebelisoa ho petsola RNA ea makhalo a le mong, RNA e nang le likhoele tse peli, le likhoele tsa RNA ho li-hybrids tsa RNA-DNA.ha e ntse e le letsoai le lengata (≥0.3 M), RNase A ka ho khetheha e ka petsola RNA ea thipa e le 'ngoe.