Proteinase K(Mokelikeli)
Melemo
● Ho ba le botsitso bo phahameng le tšebetso ea enzyme e thehiloeng ho thekenoloji e tobileng ea ho iphetola ha lintho
● E mamella letsoai la Guanidine
● RNase mahala, DNase mahala le Nickase mahala, DNA <5 pg/mg
Tlhaloso
Proteinase K ke serine protease e tsitsitseng e nang le likarolo tse pharaletseng tsa substrate.E senya liprotheine tse ngata sebakeng sa matsoalloa esita le ka pel'a li-detergents.Bopaki bo tsoang lithutong tsa kristale le sebopeho sa limolek'hule bo bontša hore enzyme ke ea lelapa la subtilisin le nang le sebaka se sebetsang sa catalytic triad (Asp 39-His 69-Ser 224).Sebaka sa mantlha sa cleavage ke bond ea peptide e bapileng le sehlopha sa carboxyl sa li-amino acid tsa aliphatic le tse nkhang hamonate tse nang le lihlopha tsa alpha amino tse koetsoeng.Hangata e sebelisoa bakeng sa tlhaloso e pharaletseng ea eona.
Sebopeho sa lik'hemik'hale
Tlhaloso
| Lintho tsa teko | Litlhaloso |
| Ponahalo(Mmala) | Ha e na mebala ho ea ho Light Brown |
| Ponahalo( Turbidity) | E Hlakile ho Hazy Haholo Hanyenyane |
| Ponahalo(Foromo) | Mokelikeli |
| Ketsahalo | ≥800U/ml |
| Protheine mg protheine /ml | ≥20 mg / ml |
| RNase | Ha ho e fumanoeng |
| DNase | Ha ho e fumanoeng |
| Nicase | Ha ho e fumanoeng |
Lisebelisoa
Setsi sa tlhahlobo ea liphatsa tsa lefutso;
RNA le lisebelisoa tsa ho ntša DNA;
Ho ntšoa ha likarolo tse seng tsa protheine ho tloha liseleng, ho senyeha ha litšila tsa protheine, joalo ka
Liente tsa DNA le ho lokisetsa heparin;
Tokisetso ea chromosome DNA ka pulsed electrophoresis;
Letlapa la Bophirimela;
Enzymatic glycosylated albumin reagents in vitro diagnostics
Thomello le Polokelo
Ho tsamaisa ka sekepe:Ambient
Maemo a polokelo:Boloka ho -20 ℃(Nako e telele)/ 2-8℃( Nako e khutšoanyane)
Letsatsi la tlhahlobo hape le khothalelitsoeng:lilemo tse 2
