dATP/ dCTP/ dGTP/ dTTP/ dUTP (100mM)
Tlhaloso
Sehlahisoa sena ke tharollo ea metsi e se nang 'mala.E loketse liteko tse fapaneng tse tloaelehileng tsa baeloji ea limolek'hule tse kang ho holisa PCR, PCR ea nako ea sebele, cDNA kapa motsoako o tloaelehileng oa DNA, tatellano ea DNA le ho ngola.E ka hlakoloa ka metsi a hloekileng haholo, 'me ea fetoloa ho pH 7.0 ka tharollo e phahameng ea NaOH, ka bohloeki ≥ 99% (HPLC).Ka mor'a ho fumanoa, ha e na DNase, RNase le phosphotase.E ka sebelisoa ka kotloloho liketsong tse fapaneng tse tloaelehileng tsa baeloji ea limolek'hule joalo ka PCR.
Sebopeho sa lik'hemik'hale
Tlhaloso
| Lintho tsa teko | Litlhaloso |
| Ponahalo | Tharollo e hlakileng e se nang mebala |
| Ho tsepamisa maikutlo | 100mM ±3% |
| Purity (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Taolo ea boleng1. Tokisetso ena ha e na tšoaetso ea DNase le RNase 2. dNTP ena e ngata e lekotsoe ts'ebetso ka Taq DNA polymerase le Pfu DNA polymerase. Boholo bona ba d NTP bo lekotsoe ts'ebetso ka Q-PCR | |
Lisebelisoa
PCR e telele (20kb)
cDNA synthesis le RT-PCR
PCR ea nako ea 'nete
PCR e tloaelehileng
PCR e tšepahalang haholo
Thomello le Polokelo
Lipalangoang:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -25～-15℃
Letsatsi la tlhahlobo hape le khothalelitsoeng:lilemo tse 2
Lintlha
1) E ka qhibiliha ka mocheso oa kamore.Ka mor'a ho qhibiliha, e lokela ho bolokoa ka lebokoseng la leqhoa kapa leqhoa.Kamora ho sebelisoa, e lokela ho bolokoa ho -25～-15 ℃ hang-hang.
2) Bakeng sa polokeho le bophelo bo botle ba hau, ka kopo roala lijase tsa lab le liatlana tse lahliloeng bakeng sa ts'ebetso.
