Mohato o le mong RT-qPCR SYBR Green Kit
Tlhaloso
Universal Blue qPCR Master Mix (Dye Based) ke tharollo ea pele bakeng sa 2 × ea sebele ea nako ea sebele ea palo ea PCR e khetholloang ka kutloisiso e phahameng le ho khetheha, e putsoa ka 'mala,' me e na le phello ea mohlala oa ho kenyelletsa mohlala.Karolo ea mantlha ea Taq DNA polymerase e sebelisa antibody e chesang ho qala ho thibela ka mokhoa o hlakileng matlafatso e sa tobang ka lebaka la "primer annealing" nakong ea tokiso ea sampole.Ka nako e ts'oanang, foromo e eketsa lintlha tse khothalletsang ho ntlafatsa katleho ea karabelo ea PCR le ho lekanya ho holisoa ha liphatsa tsa lefutso tse nang le likahare tse fapaneng tsa GC (30 ~ 70%), e le hore palo ea PCR e ka fumana kamano e ntle ea mela ka bongata. sebaka.Sehlahisoa sena se na le ROX Passive Reference Dye e khethehileng, e sebetsang ho lisebelisoa tsohle tsa qPCR.Ha ho hlokahale ho fetola khatello ea ROX ho lisebelisoa tse fapaneng.Hoa hlokahala feela ho kenyelletsa li-primers le litempele ho lokisa sistimi ea karabelo bakeng sa ho holisa.
Mokhoa oa ho itšoara
Tlhaloso
Eona | 50T |
SYBR Buffer e tala | 500 μL |
Li-enzyme | 50 μL |
50× High Rox | 50 μL |
50 × Low Rox | 50 μL |
RNase-free ddH2O | 500 μL |
Lisebelisoa
Tlhaloso ea Gene
Thomello le Polokelo
Lipalangoang:Iliphutheloana
Maemo a polokelo:Ho boloka ho -20°℃
Letsatsi la tlhahlobo hape le khothalelitsoeng:Likhoeli tse 18