Mohato o le mong RT-qPCR probe kit

One Step qRT-PCR Probe Kit e etselitsoe qPCR ka kotloloho e sebelisang RNA (mohlala virus RNA) joalo ka thempleite.Ka ho sebelisa li-primers tsa mofuta o itseng (GSP), mongolo o ka morao le qPCR li ka phethoa ka tšupu e le 'ngoe, ho fokotsa haholo mekhoa ea liphaephe le kotsi ea tšoaetso.E ka emisoa ho 55 ℃ ntle le ho ama ts'ebetso le kutlo ea qRT-PCR.Ho kopanya ts'ebetso e phahameng ea HiScript III Reverse Transcriptase le Champagne Taq DNA Polymerase e qalang e chesang, e nang le buffering system e ntlafalitsoeng, kutloisiso ea ho lemoha ha One Step qRT-PCR Probe Kit e ka fihla ho 0.1 pg ea kakaretso ea RNA kapa likopi tse ka tlase ho 10 tsa lithempleite tsa RNA. mme e na le botsitso bo phahameng ba mocheso.Mohato o le Mong qRT-PCR Probe Kit e fanoe ka Master Mix.The 5 × One Step Mix e na le buffer e ntlafalitsoeng le dNTP/dUTP Mix, 'me e loketse lisebelisoa tsa ho lemoha tse khethehileng tse thehiloeng ho li-probes tse ngotsoeng ka fluorescence (mohlala, TaqMan).