Uracil DNA Glycosylase (UDG/UNG)
Tlhaloso
Heat-Labile UDG (uracil DNA glycosylase) e thusa ho lokolloa ha uracil ea mahala ho DNA e nang le uracil ka hydrolyzing kamano ea N-glycoside pakeng tsa metheo ea uracil le skeletons ea tsoekere-phosphate.Ha ho bapisoa le li-enzyme tse tloaelehileng tsa UDG, mocheso-Labile UDG o ka qoba ho senyeha ha lihlahisoa tsa amplification tse nang le dU tse bakoang ke mosebetsi o setseng oa UDG e sa sebetseng mocheso oa kamore.Heat-Labile UDG (uracil DNA glycosylase) e sebetsa mocheso oa kamore 'me e thermolabile ebile e bonolo ho e tima.
Sebopeho sa Lik'hemik'hale
Tlhaloso
Enzyme | Glycosylase |
Buffer e lumellanang | Buffer ea polokelo |
Ho se sebetse ha mocheso | 50°C, metsotso e 10 |
Tlhaloso ea Yuniti | Yuniti e le 'ngoe (U) e hlalosoa e le palo ea enzyme e hlokahalang ho etsa hydrolysis ea 1 μg dU e nang le dsDNA ka metsotso e 30 ho 25°C. |
Lisebelisoa
1.Thuto ea tokiso ea DNA le ho lemoha liphetoho tse fetohang.
2.Eketsa katleho ea cloning ea lihlahisoa tsa PCR.
3.Eketsa katleho ea mutagenesis e tsamaisoang ke sebaka.
Thomello le Polokelo
Lipalangoang:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -15 ℃ ~ -25 ℃
Bophelo ba Shief:lilemo tse 2