Homocysteine (HCY)
Tlhaloso
Homocysteine (HCY) e sebelisetsoa ho lemoha homocysteine maling a motho.Homocysteine (Hcy) ke amino acid e nang le sebabole e hlahisoang ke metabolism ea methionine.80% ea Hcy e tlameletsoe ho liprotheine ka litlamo tsa disulfide maling, 'me ke karolo e nyenyane feela ea homocysteine ea mahala e kopanelang ho potoloha.Maemo a Hcy a amana haufi-ufi le mafu a pelo.ke ntlha ea bohlokoa ea kotsi bakeng sa lefu la pelo.Ho eketseha ha Hcy maling ho susumetsa lerako la methapo ea mali ho baka tšenyo ea methapo ea mali, e lebisang ho ruruha le ho thehoa ha plaque leboteng la sekepe, e leng se qetellang se lebisa ho thibela ho phalla ha mali pelong.Ho bakuli ba nang le hyperhomocystinuria, bofokoli bo matla ba liphatsa tsa lefutso bo ama metabolism ea Hcy, e bakang hyperhomocysteinemia.Bofokoli bo bobebe ba liphatsa tsa lefutso kapa khaello ea phepo ea livithamini tsa B li tla tsamaisana le bophahamo bo itekanetseng kapa bo bobebe ba Hcy, e leng se tla eketsa kotsi ea lefu la pelo.Hcy e phahameng e ka boela ea baka mathata a tsoalo a kang neural tube defects le congenital malformations.
Sebopeho sa Lik'hemik'hale
Molao-motheo oa teko
Oxidized Hcy e fetoloa ho Hcy ea mahala, 'me Hcy ea mahala e sebetsana le serine tlas'a catalysis ea CBS ho hlahisa L-cystathionine.L-cystathionine e hlahisa Hcy, pyruvate le NH3 tlas'a catalysis ea CBL.Piruvate e hlahisoang ke karabelo ena ea potoloho e ka bonoa ke lactate dehydrogenase LDH le NADH, 'me sekhahla sa phetoho ea NADH ho NAD se lekana ka kotloloho le litaba tsa Hcy sampoleng.
Lipalangoang le polokelo
Lipalangoang:2-8°C
Nako ea polokelo le ho sebetsa:Li-reagents tse sa buloang li lokela ho bolokoa ho 2-8 ° C lefifing, 'me nako e nepahetseng ke likhoeli tse 12;ka mor'a ho bula, li-reagents li lokela ho bolokoa lefifing ho 2-8 ° C, 'me nako e nepahetseng ke khoeli ea 1 tlas'a boemo ba ho se silafatsoa;li-reagents ha lia lokela ho hoamisoa.
Hlokomela
Litlhoko tsa lisampole: Mohlala ke serum e ncha kapa plasma (heparin anticoagulation, 0.1mg heparin e ka thibela mali a 1.0ml).Ka kopo, centrifuge plasma hang ka mor'a ho bokella mali, kapa refrigerate le centrifuge nakong ea hora e le 'ngoe.
