Glucose Dehydrogenase (GDH)
Tlhaloso
Glutamate Dehydrogenase (GDH) ke enzyme ea mitochondrial e etsang hore ho be le phetolo ea oxidative deamination ea glutamate ho a-ketoglutarate mme e sebetsa e le sehokelo sa bohlokoa lipakeng tsa anabolic le litsela tsa catabolic.Liphoofolo tse anyesang, GDH e tlas'a taolo ea allosteric mme e na le ts'ebetso e phahameng sebeteng, liphio, boko le manyeme.Ts'ebetso ea GDH ka serum e ka sebelisoa ho khetholla pakeng tsa mafu a sebete ka lebaka la ho ruruha ha sebete, e sa bontšeng mosebetsi o phahameng oa serum GDH, le mafu a bakoang ke hepatocyte necrosis, e leng se hlahisang serum e phahameng ea GDH.
Ts'ebetso ea GDH e khethoa ke tlhahlobo ea enzyme e kopantsoeng moo glutamate e jeoang ke GDH e hlahisang NADH, e sebetsanang le probe e hlahisang sehlahisoa sa colorimetric (450 nm) se lekanang le ts'ebetso ea GDH e teng.Yuniti e le 'ngoe ea GDH ke palo ea enzyme e tla hlahisa 1.0 mmole ea NADH ka motsotso pH 7.6 ho 37 °C.
Sebopeho sa Lik'hemik'hale
Mokhoa oa ho arabela
D-Glucose + e amohelang → D-Glucono-1,5-lactone + e fokolitsoeng e amohelang
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Tlhaloso | White amorphous phofo, lyophilized |
| Ketsahalo | ≥160U/mg |
| Bohloeki(SDS-PAGE) | ≥90% |
| Solubility(10 mg phofo/mL) | Hlakileng |
| Ho silafatsa li-enzyme | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Lipalangoang le polokelo
Lipalangoang: Liphutheloana tsa leqhoa
Polokelo :Boloka ho -25~-15°C(Nako e telele), 2-8°C (nako e khutšoanyane)
E khothaletsoa tlhahlobo hapeBophelo: Likhoeli tse 18
