Virusi DNA/RNA Extraction Kit
The kit(HC1009B) e ka ntša ka potlako li-nucleic acid (DNA/RNA) tse nang le bohloeki bo phahameng ho tsoa lisampoleng tse fapaneng tsa metsi tse kang mali, serum, plasma, le mokelikeli o hlatsoang oa swab, e leng se nolofalletsang ts'ebetso e phahameng ea lisampole tse tšoanang.Kit e sebelisa lifaha tsa makenete tse kentsoeng ka superparamagnetic tse thehiloeng ho silicon.Ka mokhoa o ikhethileng oa buffer, li-nucleic acid ho e-na le liprotheine le litšila tse ling li adsorbed ke li-hydrogen bond le electrostatic binding.Lifaha tsa makenete tse nang le li-nucleic acid li hlatsuoa ho tlosa liprotheine tse setseng le matsoai.Ha ho sebelisoa buffer e nang le letsoai le tlaase, li-nucleic acid li lokolloa lifaha tsa makenete, e le ho finyella morero oa karohano e potlakileng le ho hloekisa li-nucleic acid.Ts'ebetso eohle ea ts'ebetso e bonolo, e potlakile, e bolokehile ebile e sebetsa hantle, 'me li-nucleic acid tse fumanoeng li ka sebelisoa ka kotloloho bakeng sa liteko tse tlase tse kang reverse transcript, PCR, qPCR, RT-PCR, RT-qPCR, tatellano ea moloko o latelang, tlhahlobo ea biochip, etc.
Maemo a polokelo
Boloka ho 15 ~ 25 ℃, 'me u tsamaee ka mocheso oa kamore.
Lisebelisoa
Mali, serum, plasma, swab eluent, tissue homogenate le tse ling.
Mokhoa oa ho Leka
1. Mohlala ho sebetsa
1.1 Bakeng sa livaerase ka lisampole tsa mokelikeli tse kang mali, serum, le plasma: 300μL ea supernatant e sebelisetsoang ho ntšoa.
2.2 Bakeng sa disampole tsa swab: Beha lisampole tsa swab ka har'a li-tubes tsa sampole tse nang le tharollo ea "preservation", vortex bakeng sa 1 min, 'me u nke 300μL supernatant bakeng sa ho ntšoa.
1.3 Bakeng sa livaerase tse ka har'a li-tissue homogenates, litharollo tsa li-tissue soak, le lisampole tsa tikoloho: Ema lisampole metsotso e 5 -10, 'me u nke 300μL ea supernatant bakeng sa ho ntšoa.
2. Tokisetso ea prepreagent e khahlehang
Ntša li-reagents tse seng li phuthetsoe ka har'a khiti, fetola 'me u kopanye makhetlo a mangata ho emisa lifaha tsa makenete.Ka bonolo sisinya poleiti ho etsa hore li-reagents le lifaha tsa makenete li tebele botebong ba seliba.Ka kopo, netefatsa hore na poleiti e kae 'me u tabole ka hloko foil ea aluminium e koalang.
Δ Qoba ho thothomela ha o hahola filimi e koalang ho thibela mokelikeli ho tsoa.
3. Ts'ebetso ea koloiseletsa sa atic
3.1 Eketsa 300μL ea lisampole lilibeng tse Kholumong ea 1 kapa ea 7 ho tse 96 tsa poleiti e tebileng ea seliba (ela hloko boemo bo sebetsang bo sebetsang hantle).Bophahamo ba tlhahiso ea sampole bo lumellana le 100-400 μL.
3.2 Beha poleiti ea seliba se tebileng sa 96 ka har'a sesebelisoa sa li-nucleic acids.Apara matsoho a makenete, 'me u netefatse hore a koahetse lithupa tsa makenete ka botlalo.
3.3 Beha lenaneo ka tsela e latelang bakeng sa ho ntša ka mokhoa o itekanetseng:
3.4 Ka mor'a ho ntšoa, fetisetsa mokelikeli ho tloha Likholomong 6 kapa 12 tsa poleiti e tebileng ea 96 (ela hloko boemo bo sebetsang bo sebetsang hantle) ho tube e hloekileng ea Nuclease-free centrifuge.Haeba u sa e sebelise hang-hang, ka kopo boloka lihlahisoa ho -20 ℃.
Lintlha
Bakeng sa tšebeliso ea lipatlisiso feela.Ha e sebelisoe lits'ebetsong tsa tlhahlobo.
1. Sehlahisoa se nkiloeng ke DNA/RNA.Tlhokomelo e khethehileng e lokela ho lefshoa ho thibela ho senyeha ha RNA ke RNase nakong ea ts'ebetso.Lisebelisoa le li-samplers tse sebelisitsoeng li lokela ho neheloa.Lithapo tsohle le malebela a pipette li lokela ho etsoa sterilized le DNase/RNase-free.Basebelisi ba tlameha ho roala liatlana le limaske tse se nang phofo.
2. Ka kopo bala bukana ea litaelo ka hloko pele u e sebelisa, 'me u sebetse ho latela tataiso e tiileng.Ts'ebetso ea sampole e tlameha ho etsoa ka bencheng e hloekileng haholo kapa khabinete ea polokeho ea baeloji.
3. The automatic nucleic acid extract system e lokela ho bolokoa ka UV bakeng sa metsotso e 30 pele le ka mor'a tšebeliso.
4. Ho ka 'na ha e-ba le mesaletsa ea lifaha tsa makenete tse setseng sebakeng seo u se bonang ka mor'a ho ntšoa, kahoo qoba ho lakatsa lifaha tsa makenete.Haeba lifaha tsa makenete li lakatseha, li ka tlosoa ka sethala sa makenete.
5. Haeba ho se na litaelo tse khethehileng bakeng sa lihlopha tse fapaneng tsa li-reagents, ka kopo u se ke ua li kopanya, 'me u netefatse hore li-kits li sebelisoa nakong ea nako e nepahetseng.
6. Lahla lisampole tsohle le reagent hantle, hlakola ka botlalo 'me u bolaee libaka tsohle tsa mosebetsi ka 75% ea ethanol.
