Ultra Nuclease
UltraNuclease ke mofuta oa liphatsa tsa lefutso o nkiloeng ho Serratia marcescens, o khonang ho theola DNA kapa RNA, ebang ke habeli kapa e le 'ngoe, e pota-potile kapa e chitja tlas'a maemo a mangata, e senya ka ho feletseng li-nucleic acid hore e be 5'-monophosphate oligonucleotides le bolelele ba 3-5. .Ka mor'a phetoho ea liphatsa tsa lefutso, sehlahisoa se ile sa lomosoa, sa hlahisoa, 'me sa hloekisoa ho Escherichia coli (E. coli), e fokotsang ponahalo ea li-cell supernatant le cell lysate lipatlisiso tsa saense, empa hape e ntlafatsa katleho ea tlhoekiso le lipatlisiso tse sebetsang tsa protheine.E ka sebelisoa hape kalafong ea liphatsa tsa lefutso, tlhoekiso ea vaerase, tlhahiso ea ente, indasteri ea meriana ea polysaccharide e le setsi sa ho tlosa masala a nucleic acid.
Likarolo tsa sehlahisoa
| Nomoro ea CAS. | 9025-65-4 |
| Nomoro ea EC. | |
| Boima ba Molek'hule | 30kDa |
| Ntlha ea Isoelectric | 6.85 |
| Bohloeki ba Protheine | ≥99% (SDS-PAGE & SEC-HPLC) |
| Ts'ebetso e khethehileng | ≥1.1×106U/mg |
| OptimumTemperature | 37°C |
| Optimum pH | 8.0 |
| Mosebetsi oa Protease | mpe |
| Bioburden | ＜10CFU/100,000U |
| Residual Host-cell Protein | ≤10ppm |
| Tšepe e boima | ≤10ppm |
| Endotoxin ea baktheria | ＜0.25EU/1000U |
| Buffer ea polokelo | 20mM Tris-HCl, pH 8.0, 2mM MgCl2 , 20mM NaCl, 50% Glycerol |
Maemo a polokelo
≤0 ° C lipalangoang; -25~-15 ° C Ho boloka, lilemo tse 2 ho sebetsa (qoba ho qhibiliha ha leqhoa).
Tlhaloso ea Yuniti
Palo ea enzyme e sebelisitsoeng ho fetola boleng ba ho monya △A260 ka 1.0 ka har'a 30min ho 37 °C, pH 8.0, e lekanang le 37μg ea salmon ea sperm DNA ka ho seha oligonucleotides, e hlalositsoe e le yuniti e sebetsang (U).
Taolo ea boleng
Residual Host-cell Protein: ELISA kit
•Protease Mesaletsa: 250KU/mL UltraNuclease e ile ea arabela ka substrate bakeng sa 60min, ha ho mosebetsi o ileng oa fumanoa.
•Endotoxin ea baktheria: LAL-Test, Pharmacopoeia ea People's Republic of China Volume 4 (Khatiso ea 2020) mokhoa oa teko ea moeli oa gel.Melao e Akaretsang (1143).
•Bioburden: Pharmacopoeia of the People's Republic of China Volume 4 (Khatiso ea 2020)— Kakaretso
Melao ea Teko ea Botsoa (1101), PRC National Standard, GB 4789.2-2016.
•Heavy Metal:ICP-AES, HJ776-2015.
Tshebetso
Ts'ebetso ea UltraNuclease e ne e thibetsoe haholo ha mahloriso a SDS a feta 0.1% kapa EDTA
mahloriso a ne a feta 1mM. Surfactant Triton X- 100, Tween 20 le Tween 80 e ne e se na phello ho nuclease.
thepa ha mahloriso a ne a le ka tlase ho 1.5%.
| Tshebetso | Tshebetso e nepahetseng | Tshebetso e nepahetseng |
| Mocheso | 37℃ | 0-45 ℃ |
| pH | 8.0-9.2 | 6.0- 11.0 |
| Mg2+ | 1-2mM | 1-15mM |
| DTT | 0-100mM | > 100mM |
| 2-Mercaptoethanol | 0-100mM | > 100mM |
| Monovalent tšepe ion (Na+, K+ joalo-joalo) | 0-20mM | 0-200mM |
| PO43- | 0-10mM | 0-100mM |
Tšebeliso le Tekanyetso
• Tlosa exogenous nucleic acid lihlahisoa tsa ente, fokotsa kotsi ea masala a chefo ea nucleic acid le ho ntlafatsa polokeho ea sehlahisoa.
• Fokotsa viscosity ea mokelikeli oa phepelo o bakoang ke nucleic acid, khutsufatsa nako ea ho sebetsa le ho eketsa chai ea protheine.
• Tlosa nucleic acid e phuthetseng karoloana (vaerase, 'mele o kenyellelitsoeng, joalo-joalo), e loketseng.
tokollong le tlhoekisong ya phatsa.
| Mofuta oa Teko | Tlhahiso ea Liprotheine | Kokoana-hloko, Ente | Lithethefatsi Tsa Lisele |
| Nomoro ea Lisele | 1g sele e metsi boima (e emisitsoe hape ka 10ml buffer) | 1L ho belisoa mokelikeli o moholo | 1L setso |
| Bonyane Dosage | 250U | 100U | 100U |
| Recommended Dosage | 2500U | 25000U | 5000U |
• Kalafo ea nyutlelie e ka ntlafatsa tharollo le ho hlaphoheloa ha sampuli bakeng sa kholomo ea chromatography, electrophoresis le tlhahlobo ea blotting.
• Ka phekolo ea liphatsa tsa lefutso, nucleic acid e tlosoa ho fumana likokoana-hloko tse amanang le adeno tse hloekisitsoeng.
