Ultra Nuclease Assay Kit(ELISA)
Tlhaloso
Sesebelisoa sena sa Ultra Nuclease ELISA ke lehlabathe leo ELISA le lokelang ho etsoa ka sebopeho sa microplate.Mehlala eo e ka bang le endonuclease e kentsoe ka har'a liliba tsa poleiti ea microtiter tse kentsoeng pele ka "anti-endonuclease capture" e hloekileng.Ka mor'a ho qhoqhoa le mohato oa ho hlatsoa moo likarolo tse sa tlangoang li tlosoang, ho eketsoa antibody ea detector ea enzyme-conjugated, anti-endonuclease.Boitšoaro ba ho itšireletsa mafung bo fella ka ho thehoa ha sandwich complex ea solid phase antibody-endonuclease-enzyme e ngotsoeng antibody.Ka mor'a mohato oa ho qetela oa ho hlatsoa, tharollo ea substrate e kenngoa lilibeng 'me e itšoara, e fella ka nts'etsopele ea mebala.Boima ba mahlo bo lekantsoe ka mokhoa oa photometric mme bo lekana le
analyte concentration teng ka lilibeng.Khatello ea endonuclease ka lisampole tse sa tsejoeng e ka baloa ho ipapisitsoe le curve e lumellanang le eona.
Sebopeho sa Lik'hemik'hale
Tlhaloso ea Yuniti
Palo ea enzyme e sebelisitsoeng ho fetola boleng ba ho monya △A260 ka 1.0 nakong ea metsotso e 30
ka 37 °C, pH 8.0, e lekanang le DNA ea peō ea salmon ea 37μg ka ho khaola oligonucleotides, e hlalositsoe e le yuniti e sebetsang.
Tšebeliso le Tekanyetso
• Tlosa exogenous nucleic acid lihlahisoa tsa ente, fokotsa kotsi ea masala a chefo ea nucleic acid le ho ntlafatsa polokeho ea sehlahisoa.
• Fokotsa viscosity ea mokelikeli oa phepelo o bakoang ke nucleic acid, khutsufatsa nako ea ho sebetsa le ho eketsa chai ea protheine.
• Tlosa nucleic acid e neng e phuthetsoe ka karoloana (vaerase, 'mele oa ho kenyelletsa, joalo-joalo), e leng se loketseng ho lokolloa le ho hloekisa karoloana.
• Kalafo ea nyutlelie e ka ntlafatsa tharollo le ho hlaphoheloa ha sampuli bakeng sa kholomo ea chromatography, electrophoresis le tlhahlobo ea blotting.
• Ka phekolo ea liphatsa tsa lefutso, nucleic acid e tlosoa ho fumana likokoana-hloko tse amanang le adeno tse hloekisitsoeng.
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Moeli o tlase oa ho lemoha | 0.6 ng/mL |
| Moeli o tlase oa bongata | 0.2 ng/mL |
| Nepahalo | Intra assay CV≤10% |
Lipalangoang le polokelo
Lipalangoang:E rometsoe ka tlas'a 0 °C
Polokelo:Boloka ho -2-8 ° C, e butsoe reagent e tsitsitseng bakeng sa libeke tse 6
E khothalelitsoe ho lekoa hape Bophelo:1 selemo
