Phosphatase Alkaline (ALP)
Tlhaloso
Alkaline Phosphatase e tsoa mofuteng oa motsoako oa E. coli o jereng lefutso la TAB5.Enzyme e susumetsa dephosphorylation ea 5' le 3' lipheletsong tsa DNA le RNA phosphomonoesters.Hape, e etsa hydrolyses ribose, hammoho le deoxyribonucleoside triphosphates (NTPs le dNTPs).TAB5 Alkaline Phosphatase e sebetsa ho 5' ho hlahella, 5' ho khutlela morao le lipheletsong tse lerootho.Phosphatase e ka sebelisoa lits'ebetsong tse ngata tsa baeloji ea limolek'hule, joalo ka cloning kapa probe end label ho tlosa lipheletso tsa phosphorylated tsa DNA kapa RNA.Litekong tsa cloning, dephosphorylation e thibela DNA ea plasmid ea linearized hore e se ke ea itšehla thajana.E ka boela ea theola li-dNTP tse sa kenyelletsoeng karabelong ea PCR ho lokisa template bakeng sa tatellano ea DNA.Enzyme ha e sebetse ka botlalo ebile e ke keng ea fetoloa ka ho futhumatsa ho 70 ° C metsotso e 5, ka hona, ho tlosoa ha phosphatase pele ho ligation kapa ho emisa ho ngola ho sa hlokahale.
Tšebeliso
1.Alkaline phosphatase e kopantsoeng le liprotheine (li-antibodies, streptavidin joalo-joalo,) li ka khetholla ka ho khetheha limolek'hule tsa sepheo, 'me li ka sebelisoa ho ELISA, WB le ho lemoha histochemical;
2.Alkaline phosphatase e ka sebelisoa ho dephosphorize 5 '-terminal ea DNA kapa RNA ho thibela ho itšehla thajana;
3.Dephosphorylated DNA kapa RNA e ka holimo e ka ngoloa ke phosphates e ngotsoeng ka seea-le-moea (ka T4 poly-nucleotide kinase)
Sebopeho sa Lik'hemik'hale
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Ts'ebetso ea enzyme | 5U/μL |
| Ts'ebetso ea Endonuclease | Ha e fumanwe |
| Ts'ebetso ea Exonuclease | Ha e fumanwe |
| Ts'ebetso ea Nicking | Ha e fumanwe |
| Mosebetsi oa RNase | Ha e fumanwe |
| E.coli DNA | ≤1kopi/5U |
| Endotoxin | Tlhahlobo ea LAL, ≤10EU/mg |
| Bohloeki | ≥95% |
Lipalangoang le polokelo
Lipalangoang:AmbIent
Polokelo :Boloka mocheso oa 2-8 ° C
E khothaletsoa tlhahlobo hapeBophelo:2 lilemo
