Hexokinase (HK)
Tlhaloso
Sebelisa Hexokinase bakeng sa qeto ea D-glucose, D-fructose, le D-sorbitol lijong kapa lisampoleng tsa lipatlisiso tsa baeloji.Enzyme e boetse e sebelisoa bakeng sa tlhahlobo ea li-saccharide tse ling tse fetolehang ho tsoekere kapa fructose, ka hona e thusa tlhahlobong ea li-glycoside tse ngata.
Haeba Hexokinase e sebelisoa hammoho le glucose-6-phosphate dehydrogenase (G6P-DH)* (litlhahlobo tsa glucose6-phosphate e entsoeng ke Hexokinase), lisampole ha lia lokela ho ba tsa phosphate concentrations e ngata kaha G6P-DH e thibetsoe ka tlholisano ke phosphate.
Sebopeho sa Lik'hemik'hale
Molao-motheo oa ho itšoara
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Tlhaloso | E tšoeu ho isa ho mosehla o monyane phofo ea amorphous, lyophilized |
| Ketsahalo | ≥30U/mg |
| Bohloeki(SDS-PAGE) | ≥90% |
| Solubility(10mg phofo/ml) | Hlakileng |
| Maprotease | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-Phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Lipalangoang le polokelo
Lipalangoang: Ambient
Polokelo :Boloka ho -20°C(Nako e telele), 2-8°C (nako e khutšoanyane)
E khothaletsoa tlhahlobo hapeBophelo:1 selemo
