Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase ke qalo e chesang ea DNA polymerase e nang le thibelo e habeli ka li-antibodies tse peli.Sehlahisoa sena ha se thibe feela mosebetsi oa 5′→3′ polymerase oa Taq DNA polymerase, empa hape o thibela mosebetsi oa 5′→3′exonuclease.Ho futhumatsa metsotsoana e 30 mocheso oa pele ho denaturation ho ka etsa hore antibody ka ho feletseng 'me e lokolle mosebetsi oa DNA polymerase le mosebetsi oa exonuclease.Tšobotsi ea ho thibela habeli e ke ke ea thibela feela ho holisoa ho sa hlakang ho bakoang ke ho se lumellane kapa primer dimer, empa hape ho thibela ka nepo phokotso ea lets'oao la fluorescence le bakoang ke ho senyeha ha probe, e le ho etsa hore reagent ea ho lemoha in vitro e tsitsitse nakong ea lipalangoang kapa e sebelisoa ka phapusing. mocheso.
Likaroloana
| Karolo | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq DNA Polymerase(5 U/μL) | 50 μL | 200 μL | 2 mL | 5 mL |
Boemo ba polokelo
Sehlahisoa se romelloa ka leqhoa le omileng 'me se ka bolokoa ho -25 ° C ~ -15 ° C ka lilemo tse 2.
Litlhaloso
| Polymerase | Taq DNA Polymerase |
| Bohloeki | ≥ 95% (SDS-PAGE) |
| Hot Qala | E hahiloeng ka Hot Start |
| Lebelo la ho arabela | Standard |
| Ts'ebetso ea Exonuclease | 5′→3′ |
Litaelo
Rection Setup
| Likaroloana | Bolumo (μL) | Boikutlo ba ho Qetela |
| 2 × Buffera | 25 | 1× |
| Motsoako oa Primer/Probeb | × | 0.1 μmol/L-0.5 μmol/L |
| Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| Setšoantšo sa DNAc | × | 0.1-100 ng |
| ddH2O | Ho fihlela ho 50 | - |
Lintlha:
1) Ho latela ts'ebeliso e ikhethileng ea liteko, hoa hlokahala ho lokisa "buffer" e ts'oanang.
2) Palo ea DNA le mahloriso a li-probes kapa li-primers li khothaletsoa ho tsepamisa maikutlo.Mahloriso a nepahetseng a ka fetoloa ho latela maemo a khethehileng a liteko.
Thermal cycling protocol
| Mohato | Mocheso(°C) | Nako | Lipotoloho |
| Pre-denaturation | 95 ℃ | 5 mets | 1 |
| Denaturation | 95 ℃ | 15 metsotsoana | 45 |
| Anealing/ Ketsetso | 60 ℃a | 30 metsotsoanab |
Lintlha:
1) Mocheso oa karabelo o lokisoa ho latela boleng ba Tm ba li-primers tse etselitsoeng.
2) Lisebelisoa tse fapaneng tsa qPCR li hloka nako e fapaneng ea ho fumana matšoao a fluorescence, ka kopo beha ho latela nako e khuts'oane.
Lintlha
Ka kopo, roala PPE e hlokahalang, jase e joalo ea lab le liatlana, ho netefatsa bophelo bo botle le polokeho ea hau!
