DNase I (Rnase Free)(5u/ul)
Nomoro ea katse: HC4007A
DNase I (Deoxyribonuclease I) ke endodeoxyribonuclease e ka silang DNA e nang le mela e le 'ngoe kapa e habeli.E lemoha le ho qhetsola libond tsa phosphodiester ho hlahisa monodeoxynucleotides kapa oligodeoxynucleotide e nang le stranded e le 'ngoe kapa habeli ka lihlopha tsa phosphate ho 5'-terminal le hydroxyl ho 3'-terminal.Mosebetsi oa DNase I o itšetlehile ka Ca2+'me e ka kenngoa ts'ebetsong ke li-ion tsa divalent tsa tšepe tse kang Mn2+le Zn2+.5 mM Ca2+e sireletsa enzyme ho tloha hydrolysis.Boteng ba Mg2+, enzyme e ne e khona ho lemoha le ho petsola sebaka leha e le sefe moleng ofe kapa ofe oa DNA.Ka pel’a Mon2+, likhoele tse peli tsa DNA li ka hlokomeloa ka nako e le ’ngoe ’me tsa petsoha hoo e batlang e le sebakeng se le seng ho etsa likaroloana tsa DNA tse sephara kapa likhechana tsa DNA tse khomarelang tse nang le li-nucleotide tse 1-2 tse hlaheletseng.
Likaroloana
| Lebitso | 0.1KU | 1KU | 5 KU | 50 KU |
| DNase I, RNase-mahala | 20μL | 200μL | 1mL | 10 ml |
| 10×DNase I Buffer | 1mL | 1mL | 5 × 1mL | 5 × 10 mL |
Maemo a polokelo
-25 ℃~-15 ℃ bakeng sa polokelo;Lipalangoang tlas'a leqhoa.
Litaelo
1. Lokisetsa tharollo ka har'a tube e se nang RNase ho latela litekanyo tse thathamisitsoeng ka tlase:
| Karolo | Bolumo |
| RNA | Xµg |
| 10 × DNase I Buffer | 1μL |
| DNase I, RNase-mahala(5U/μL) | 1 U ka µg RNA① |
| ddH2O | Ho fihlela ho 10μL |
Tlhokomeliso: ① Bala palo ea DNase I e hlokang ho eketsoa ho latela palo ea RNA.
2. 37 ℃ bakeng sa metsotso e 15;
3. Kenya 0.5M EDTA sebakeng sa ho qetela sa 2.5mM~5mM, 'me u chese ho 65℃ metsotso e 10 ho emisa karabelo.Mohlala o ka sebelisoa ka kotloloho bakeng sa karabelo e latelang joalo ka mongolo oa reverseteko.
Tlhaloso ea Yuniti
Yuniti e le 'ngoe e hlalosoa e le palo ea enzyme e tla theola 1µg ea pBR322 ka botlaloDNA ka metsotso e 10 ho 37 ℃.
Taolo ea Boleng
RNase:5U ea DNase I e nang le 1.6µg MS2 RNA bakeng sa lihora tse 4 ho 37 ℃ ha e hlahise ho senyeha hae khethoa ke agarose gel electrophoresis.
Lintlha
1. Ka kopo, lokisetsa 0.5MEDTA u le mong.
2. Sebelisa 1U DNase I ka µg ea RNA.Leha ho le joalo, haeba RNA e le ka tlase ho 1µg, ka kopo sebelisa 1U DNase I.
3. Ka kopo, beha enzyme holim'a leqhoa nakong ea ts'ebetso.
