Cholesterol oxidase(COD/CHOD)
Tlhaloso
Cholesterol oxidase (CHOD) e hlohlelletsa mohato oa pele oa k'holeseterole ea catabolism.Libaktheria tse ling tse se nang pathogenic, joalo ka Streptomyces, li khona ho sebelisa k'holeseterole joalo ka mohloli oa khabone.Libaktheria tsa pathogenic, joalo ka Rhodococcus equi, li hloka hore CHOD e tšoaetse macrophage ea moamoheli.CHOD ha e sebetse ka bobeli.Cholesterol e qala oxidized ho cholest-5-en-3-one ka mohato o hlokang FAD.Cholest-5-en-3-one e isomerized ho cholest-4-en3-one. Boitšoaro ba isomerization bo ka fetoha ka mokhoa o itseng.Mosebetsi oa CHOD o itšetlehile ka thepa ea 'mele ea membrane eo substrate e tlamisoang ho eona.
CHOD e sebelisoa ho khetholla serum cholesterol.Ke enzyme ea bobeli e sebelisoang haholo lits'ebetsong tsa tlhahlobo ka mor'a glucose oxidase.CHOD e boetse e fumana ts'ebetso ho microanalysis ea steroid ka lisampole tsa lijo le ho khetholla 3-ketosteroids ho tloha 3b-hydroxysteroids. Limela tsa Transgenic tse hlalosang k'holeseterole oxidase li ntse li batlisisoa ntoeng khahlanong le kokoana ea k'hothone.Cholesterol oxidase le eona e 'nile ea sebelisoa e le lipatlisiso tsa limolek'hule ho hlakisa likarolo tsa lisele tsa cellular.
Sebopeho sa Lik'hemik'hale
Molao-motheo oa ho itšoara
Cholesterol + O2 →△4-Cholesten-3-one + H2O2
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Tlhaloso | Yellow amorphous phofo, lyophilized |
| Ketsahalo | ≥8U/mg |
| Bohloeki(SDS-PAGE) | ≥90% |
| Solubility(10mg phofo/ml) | Hlakileng |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Esterase ea k'holeseterole | ≤0.01% |
| ATPase | ≤0.005% |
Lipalangoang le polokelo
Lipalangoang:E rometsoe ka sekepe ka tlase ho -15 ° C
Polokelo :Boloka ho -25~-15°C(Nako e telele), 2-8°C(Nako e khutšoanyane)
E khothaletsoa tlhahlobo hapeBophelo:1 selemo
