Bst 2.0 DNA Polymerase (e se nang Glycerol, e phahameng haholo)
Bst DNA polymerase V2 e tsoa ho Bacillus stearothermophilus DNA Polymerase I, e nang le ts'ebetso ea 5′→3' DNA polymerase le ts'ebetso e matla ea ketane, empa ha ho na 5′→3′ exonuclease.Bst DNA Polymerase V2 e loketse hantle bakeng sa ho falla ha strand, isothermal amplification LAMP (Loop mediated isothermal amplification) le tatellano e potlakileng.Bst DNA polymerase V2 ena e khona ho thibela ts'ebetso ea DNA polymerase mochesong oa kamore, e le hore e ka sebetsoa mme sistimi ea karabelo e ka etsoa ka mocheso oa kamore, ho thibela ho holisoa ho sa hlakang le ho ntlafatsa ts'ebetso ea karabelo, mme mofuta ona o ka etsoa. ho ba lyophilized.Ho phaella moo, e khona ho lokolla mosebetsi oa eona ka mocheso o phahameng, kahoo e felisa tlhokahalo ea mohato o fapaneng oa ts'ebetso.
Likaroloana
| Karolo | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (e se nang Glycerol) (32U/μL) | 0.05 mL | 0.25 mL | 2.5 mL |
| 10 × HC Bst V2 Buffer | 1.5 mL | 2×1.5 mL | 3 × 10 mL |
| MgSO4 (100mM) | 1.5 mL | 2×1.5 mL | 2 × 10 mL |
Lisebelisoa
1.Ntlafatso ea motlakase ea LAMP
2.DNA strand single displacement reaction
3.Tatelano ea liphatsa tsa lefutso tse phahameng tsa GC
4.Tatelano ea DNA ea boemo ba nanogram.
Boemo ba polokelo
Lipalangoang ka tlas'a 0°C 'me li bolokoe ho -25°C~-15°C.
Tlhaloso ea Yuniti
Yuniti e le 'ngoe e hlalosoa e le palo ea enzyme e kenyelletsang 25 nmol ea dNTP linthong tse sa keneng tsa asiti ka metsotso e 30 ho 65 ° C.
LEMPA tsiboso
| Likaroloana | 25 μLTsamaiso |
| 10 × HC Bst V2 Buffer | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTPs (10mM e 'ngoe le e 'ngoe) | 3.5 μL |
| SYTO™ 16 e tala (25×)a | 1.0 μL |
| Motsoako oa peleb | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-mahala) (32 U/uL) | 0.25 μL |
| Setšoantšo | × μL |
| ddH₂O | Ho fihlela ho 25 μL |
Lintlha:
1) a.SYTOTM 16 Green (25 ×): Ho ea ka litlhoko tsa liteko, li-dyes tse ling li ka sebelisoa e le li-substitutes;
2) b.Primer mix: e fumanwe ka ho kopanya 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB le mefuta e meng.
Boitšoaro le Boemo
1 × HC Bst V2 Buffer, mocheso oa incubation o pakeng tsa 60°C le 65°C.
Ho se sebetse ha mocheso
80°C, metsotso e 20.
