Viral DNA / RNA Extraction Kit
Setsi sena se loketse ho ntšoa ka potlako ha DNA/RNA ea kokoana-hloko e phahameng ho tloha lisampoleng tse kang nasopharyngeal swabs, li-swabs tsa tikoloho, li-cell culture supernatants, le li-tissue homogenate supernatants.Theknoloji e thehiloe ho thekenoloji ea ho hloekisa lera la silica e felisang tlhokahalo ea ho sebelisa phenol / chloroform organic solvents kapa nako e telele ea tahi e nang le metsi ho ntša DNA / RNA ea kokoana-hloko ea boleng bo phahameng.Li-nucleic acid tse fumanoeng ha li na litšila 'me li loketse ho sebelisoa litekong tse tlase tse kang reverse transcript, PCR, RT-PCR, PCR ea nako ea sebele, tatellano ea moloko o latelang (NGS), le Northern blot.
Maemo a polokelo
Boloka ho 15 ~ 25 ℃, 'me u tsamaee ka mocheso oa kamore
Likaroloana
| Likaroloana | 100RXNS |
| Buffer VL | 50 ml |
| Buffer RW | 120 ml |
| RNase-free ddH2 O | 6 ml |
| Litšiea tsa FastPure RNA | 100 |
| Lithapo tsa Pokello (2ml) | 100 |
| Mahala a Pokello a RNase(1 .5ml) | 100 |
Buffer VL:Fana ka tikoloho bakeng sa lysis le ho tlama.
Buffer RW:Tlosa liprotheine tse setseng le litšila tse ling.
RNase-free ddH2O:Elute DNA/RNA ho tloha lera ka har'a kholomo ea spin.
Litšiea tsa FastPure RNA:Haholo-holo adsorb DNA/RNA.
Li-tubes tsa pokello 2 ml:Bokella filtrate.
Mahala a Pokello a RNase 1.5 ml:Bokella DNA/RNA.
Lisebelisoa
Li-swabs tsa nasopharyngeal, li-swabs tsa tikoloho, li-supernatants tsa cell culture, le li-tissue homogenate supernatants.
Boiketsi ba Materials
Malebela a se nang RNase pipette, 1.5 ml RNase-free centrifuge tubes, centrifuge, vortex mixer, le pipettes.
Mokhoa oa ho Leka
Etsa mehato eohle e latelang ka har'a khabinete ea biosafety.
1. Eketsa 200 μl ea sampole ho RNase-free centrifuge tube (etsa ka PBS kapa 0.9% NaCl ha ho sa lekana sampole), eketsa 500 μl ea Buffer VL, kopanya hantle ka vortexing bakeng sa 15 - 30 sec, le centrifuge. ka bokhuts'oane ho bokella motsoako ka tlase ho tube.
2. Beha Litšiea tsa FastPure tsa RNA ka har'a Li-tubes tsa Pokello 2 ml.Fetisetsa motsoako ho tloha Mohato oa 1 ho ea FastPure RNA Columns, centrifuge ho 12,000 rpm (13,400 × g) bakeng sa 1 min, 'me u lahle filtrate.
3. Kenya 600 μl ea Buffer RW ho FastPure RNA Columns, centrifuge ho 12,000 rpm (13,400 × g) bakeng sa metsotsoana e 30, 'me u lahle filtrate.
4. Pheta Mohato oa 3.
5. Centrifuge kholomo e se nang letho ho 12,000 rpm (13,400 × g) bakeng sa 2 min.
6. Fetisetsa ka hloko Likholomo tsa FastPure RNA ho Li-Tubes tsa Pokello tse sa lefelloeng tsa RNase 1.5 ml (e fanoeng ka har'a kit), 'me u kenye 30 - 50 μl ea RNase-free ddH2O bohareng ba lera ntle le ho ama kholomo.Lumella ho ema mocheso oa kamore bakeng sa 1 min le centrifuge ho 12,000 rpm (13,400 × g) bakeng sa 1 min.
7. Lahla Litšiea tsa FastPure RNA.DNA / RNA e ka sebelisoa ka ho toba bakeng sa litlhahlobo tse latelang, kapa ho bolokoa ho -30 ~ -15 ° C ka nako e khutšoanyane kapa -85 ~ -65 ° C nako e telele.
Lintlha
Bakeng sa tšebeliso ea lipatlisiso feela.Ha e sebelisoe lits'ebetsong tsa tlhahlobo.
1. Lokisa lisampole ho mocheso oa kamore esale pele.
2. Livaerase li tšoaetsanoa haholo.Ka kopo netefatsa hore mehato eohle ea tšireletso e hlokahalang e nkuoa pele ho teko.
3. Qoba ho qhoqhoa khafetsa le ho qhibiliha ha sampuli, kaha sena se ka lebisa ho senyeha kapa ho fokotsa chai ea kokoana-hloko e nkiloeng DNA / RNA.
4. Lisebelisoa tse itokiselitseng li kenyelletsa malebela a pipette a RNase-free, 1.5 ml RNase-free centrifuge tubes, centrifuge, vortex mixer, le pipettes.
5. Ha u sebelisa khiti, roala jase ea lab, liatlana tsa latex tse lahloang, le maske a ka lahloang, 'me u sebelise lisebelisoa tse se nang RNase ho fokotsa kotsi ea tšoaetso ea RNase.
6. Etsa mehato eohle mochesong oa kamore ntle le ha ho boletsoe ka tsela e 'ngoe.
Mokhoa le Tsamaiso ea Mosebetsi
