Uracil DNA Glycoylase (e se nang Glycerol)
Tlhaloso
Thermosensitive UDG (uracil-DNA glycosylase) e ka etsa hore ho be le hydrolysis ea uracil base ea ketane ea DNA e nang le uracil le N-glycosidic bond ea mokokotlo oa tsoekere-phosphate ho lokolla uracil ea mahala.Ha ho bapisoa le li-enzyme tse tloaelehileng tsa UDG, li-enzyme tsa UDG tsa thermosensitive li qoba ts'ebetso e ka bang teng ea li-enzyme tse tloaelehileng tsa UDG kamora ho se sebetse, tse ka senyang lihlahisoa tsa amplification tse nang le dU mocheso oa kamore.Sehlahisoa sena se sebetsa mochesong oa kamore 'me se na le mocheso o bonolo ebile se atisa ho tima.
Sebopeho sa Lik'hemik'hale
Tlhaloso
| Enzyme | Glycosylase |
| Buffer e lumellanang | Buffer ea polokelo |
| Ho se sebetse ha mocheso | 50°C, metsotso e 10 |
| Tlhaloso ea Yuniti | Yuniti e le 'ngoe (U) e hlalosoa e le palo ea enzyme e hlokahalang ho etsa hydrolysis ea 1 μg dU e nang le dsDNA ka metsotso e 30 ho 25°C. |
Lisebelisoa
Tlosa tšilafalo ea aerosol e nang le PCR e nang le dU.
Ho tlosoa ha metheo ea uracil ho DNA e nang le likhoele tse peli kapa tse peli
Thomello le Polokelo
Lipalangoang:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -15 ℃ ~ -25 ℃
Bophelo ba Shief:1 lilemo
