Taq DNA Anti-'Mele
Taq DNA Antibody ke antibody e thibelang Taq DNA Polymerase monoclonal bakeng sa ho qala PCR e chesang.E ka thibela ts'ebetso ea 5′→3′ polymerase le 5′→3′ exonuclease ka mor'a ho tlama Taq DNA Polymerase, e ka thibelang ka mokhoa o sa tobang ho ts'oaroa ha li-primers le ho holisoa ho sa hlaka ho bakoang ke primer dimer ka mocheso o tlase.Ho phaella moo, sehlahisoa se ka thibela ho senyeha ha probe ka katleho.Taq DNA Antibody e hlahisoa mohatong oa pele oa DNA denaturation ea karabelo ea PCR, eo ka eona ts'ebetso ea DNA polymerase e khutlisoang ho fihlela phello ea ho qala PCR e chesang.E ka sebelisoa tlas'a boemo ba karabelo ea PCR ntle le ts'ebetso e khethehileng ea antibody.
Boemo ba polokelo
Sehlahisoa se romelloa ka lipakete tsa leqhoa 'me se ka bolokoa ho -25 ° C ~ -15 ° C ka lilemo tse 2.
Lisebelisoa
Boima ba sehlahisoa sena ke 5 mg/mL.1 μL antibody e ka thibela ts'ebetso ea 20-50 U Taq DNA polymerase.Ho kgothaletswa ho kopanya antibody le Taq DNA polymerase mochesong oa kamore bakeng sa hora e le 1 (e kenya mocheso oa kamore bakeng sa lihora tse 2 ha molumo o feta 200 mL, 'me moreki o lokela ho fetola ts'ebetso ha a sebelisoa molumo o moholoanyane), ebe o boloka. ho -20 ℃ bosiung bo bong pele o sebelisoa.
Tlhokomeliso: Ts'ebetso e ikhethileng ea Taq DNA Polymerase e fapaneng e fapane, tekanyo ea ho thibela e hloka ho lokisoa ka nepo ho fihlela katleho ea ho thibela e betere ho feta 95%.
Litlhaloso
Karolelano | Monoclonal |
Mofuta | Sethibela mmele |
Antigen | Taq DNA Polymerase |
Sebopeho | Mokelikeli |
Lintlha
Ka kopo, roala PPE e hlokahalang, jase e joalo ea lab le liatlana, ho netefatsa bophelo bo botle le polokeho ea hau!