RT-LAMP Colormetric (bolo ea Lyophilized)

Sehlahisoa sena se na le "reaction buffer", RT-Enzymes Mix (Bst DNA polymerase le reverse transcriptase e thibelang mocheso), li sireletsang lyophilized le likarolo tsa dae tsa chromogenic.Sehlahisoa ke mofuta oa bolo o nang le lyophilized, o sebelisoa feela ka li-primers le li-templates.Setsi sena se fana ka pono e potlakileng, e hlakileng ea pono ea amplification, e leng karabelo e mpe e bontšoang ka karabelo e khubelu le e ntle e bontšoa ke phetoho ho mosehla.

Karolo

RT-LAMP Colormetric Master Mix (Lifaha tsa Lyophilized)

Lisebelisoa

Bakeng sa DNA kapa RNA isothermal amplification.

Maemo a polokelo

E tsamaisoa le ho bolokoa ho 2 ~ 8 ℃.Sehlahisoa se sebetsa bakeng sa likhoeli tse 12.

Protocol

1.Ntša palo e tsamaellanang ea Lyophilized lifaha phofo ho ea ka palo ea liteko.

2.Lokisetsa motsoako oa maikutlo

Lintlha:

1. 10 × Primer Mix Concentration: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;

2. Li-template tsa Nucleic acid li khothalletsoa hore li qhibilihe ka metsi a DEPC.

3.Eketsa ho 65°C bakeng sa 30-45mins, e ka atoloswa ka nepo ho ya ka phetoho ya mmala Nako ya Rection.

4.Ho ea ka leihlo le hlobotseng, mosehla o ne o le motle 'me o mofubelu o ne o le mpe.

Lintlha

1.Mocheso oa karabelo o ka ntlafatsoa lipakeng tsa 62 ℃ le 68 ℃ ho latela boemo ba pele.

2.Li-reagents tse pakiloeng ha lia lokela ho pepesetsoa moea nako e telele.

3.Karabelo ea 'mala o mofubelu le o mosehla o ipapisitse le phetoho ea pH ea sistimi ea karabelo, ka kopo u se ke oa sebelisa tharollo ea polokelo ea Tris nucleic acid, e khothalletsoang ho sebelisa ddH.₂O bolokoa nucleic acid.

4.Teko e tla etsoa ka mokhoa o tloaelehileng, ho kenyeletsoa ho lokisoa ha sistimi ea karabelo, kalafo ea mohlala le tlatsetso ea sampole.

5.Ho khothaletsoa hore ho lokisetsoe sistimi ea karabelo tafoleng e hloekileng haholo le ho kenya litempele ka har'a mosi oa likamore tse ling ho qoba leshano.