Ribonuclease III (RNase III)

EC 3.1.24

Bohloeki.: 2U/μL

Sephutheloana: 50μL, 125μL

Lintlha tsa Sehlahisoa

Tlhaloso

Sehlahisoa sena ke ribonuclease III (RNase III) e hlahisitsoeng hape ke E. coli.Exonuclease ena e ikhethileng e arola RNA (dsRNA) e marako a mabeli 'me e hlahisa likotoana tsa 12-35bp dsRNA tse nang le 5'-PO4 le 3'-OH, 3' overhangs.

Sebopeho sa Lik'hemik'hale

Tlhaloso ea Yuniti

Tlhaloso ea yuniti ea tšebetso: Yuniti e le 'ngoe e bolela palo ea enzyme e hlokahalang ho theola 1 μg ea
dsRNA ho siRNA ho 50 μL reaction system ho 37°C metsotso e 20.

Tlhaloso

Lintho tsa Tlhahlobo	Litlhaloso
Ts'ebetso ea Exonuclease	E lokolloa ＜ 0.1% ea kakaretso ea radioactivity
Ketso e sa Totobalang ea Dnase	Ha e bonahale
Protein Purity Assay(SDS-PAGE)	≥ 95%
Ts'ebetso ea RNase (Tšelo e Atolositsoeng)	90% ea karoloana ea RNA e lula e tiile