DNase I (Rnase Free) (2u/ul)
Nomoro ea katse: HC4007B
DNase I ke endonuclease e khonang ho sila DNA e nang le mela e 'meli.E ka hydrolyze phosphodiester litlamo ho hlahisa mono-le oligodeoxynucleotides e nang le sehlopha sa 5'-phosphate le sehlopha sa 3'-OH.Mokhoa o nepahetseng oa ho sebetsa oa pH oa DNase I ke 7-8.Mosebetsi oa DNase I o itšetlehile ka Ca2+'me e ka kenngoa ts'ebetsong ke li-ion tsa tšepe tsa divalent tse kang CO2, Mon2+, Zn2+, joalo-joalo Boteng ba Mg2+, DNase Ke khona ho petsola sebaka leha e le sefe sa DNA e nang le mefuta e 'meli;Ha a ntse a le ka pel’a Mon2+, DNase I ke khona ho petsola DNA e likhoele habeli sebakeng se le seng, ea etsa lipheletso tse lerootho kapa tse khomarelang tse nang le li-nucleotide tse 1-2 tse hlahelletseng.E ka sebelisoa bakeng sa ho sebetsana le lisampole tse fapaneng tsa RNA.
Likaroloana
| Lebitso | 1KU | 5KU |
| Recombinant DNaseI (RNase-mahala) | 500 μL | 5 × 500 μL |
| DNase I Reaction Buffer (10×) | 1 mL | 5 × 1mL |
Maemo a polokelo
Sehlahisoa sena se lokela ho bolokoa ho -25 ~ -15 ℃ ka lilemo tse 2.Ka kopo qoba ho qhibiliha khafetsa.
Litaelo
E sebelisoa ha ho ntšoa DNA ho lisampole tsa RNA bakeng sa litšupiso feela.
1. Ka kopo sebelisa liphaephe tsa centrifuge tse se nang RNase le malebela a pipette ho lokisa mokhoa o latelang oa ho arabela:
| Likaroloana | Bolumo (μL) |
| DNase I Reaction Buffer (10×) | 1 |
| Recombinant DNasel (RNase-mahala) | 1 |
| RNA | X |
| RNase-free ddH2O | Ho fihlela ho 10 |
2. Maemo a ho itšoara ka tsela e latelang: 37 ℃, ka mor'a metsotso ea 15-30, eketsa motsoako oa ho qetela oa 2.5 mM EDTA tharollo 'me u kopanye hantle, ebe 65 ℃ bakeng sa 10 mins.Setšoantšo se sebelitsoeng se ka sebelisoa bakeng sa liteko tse latelang tsa RT-PCR kapa RT-qPCR, joalo-joalo.
Lintlha
1. DNase l e na le kutloelo-bohloko ho denaturation ea 'mele;Ha u kopanya, ka bonolo fetola tube ea teko lesisinyeha hantle, o seke wa sisinyeha ka matla.
2. Enzyme e lokela ho bolokoa ka lebokoseng la leqhoa kapa holim'a bate ea leqhoa ha e sebelisoa, 'me e lokela ho bolokoa ho -20 ℃ hang ka mor'a ho sebelisoa.
3. Sehlahisoa sena ke sa tšebeliso ea lipatlisiso feela.
4. Ka kopo sebetsa ka lijase tsa lab le liatlana tse lahloang, molemong oa polokeho ea hau.
