Peroxidase (Horseradish mohloli) Synonym: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) e arohane le metso ea horseradish (Amoracia rusticana) 'me ke ea sehlopha sa ferroprotoporphyrin sa peroxidase.HRP e kopana habonolo le hydrogen peroxide (H2O2).Sephetho sa [HRP-H2O2] se rarahaneng se ka oxidize mefuta e mengata e fapaneng ea bafani ba haedrojene:
Mofani + H2O2 → Mofani oa Oxidized + 2 H2O
HRP e tla oxidize substrates tse fapaneng (sheba Lethathamo la 1):
• Chromogenic
• Chemiluminescent (jwalo ka luminol kapa isoluminol)
• Fluorogenic (e kang tyramine, homovanillic acid, kapa 4-hydroxyphenyl acetic acid)
HRP ke polypeptide e le 'ngoe ea ketane e nang le marokho a mane a disulfide.HRP ke glycoprotein e nang le 18% ea lik'habohaedreite.Sebopeho sa lik'habohaedreite se na le galactose, arabinose, xylose, fucose, mannose, mannosamine le galactosamine, ho itšetlehile ka isozyme e khethehileng.

HRP ke leibole e sebelisoang haholo bakeng sa li-immunoglobulins lits'ebetsong tse ngata tse fapaneng tsa immunochemistry, ho kenyeletsoa immunoblotting, immunohistochemistry, le ELISA.HRP e ka kopanngoa le li-antibodies ka mekhoa e mengata e fapaneng, ho kenyelletsa glutaraldehyde, periodate oxidation, ka li-bond tsa disulfide, hape ka li-amino le thiol tse lebisitsoeng li-cross-linkers.HRP ke leibole e lakatsehang ka ho fetisisa bakeng sa masole a 'mele, kaha ke eona e nyenyane ka ho fetisisa le e tsitsitseng ka ho fetisisa ho tse tharo tse tummeng ka ho fetisisa tsa li-enzyme (peroxidase, β-galactosidase, alkaline phosphatase) 'me glycosylation ea eona e lebisa ho tlase ho tlamahane ho seng ka ho khetheha.Ho hatisitsoe tlhahlobo ea glutaraldehyde le mekhoa ea ho kopanya nako le nako.

Peroxidase e boetse e sebelisoa bakeng sa ho lekanya tsoekere ea 4 le peroxides ka tharollo.Lingoliloeng tse ngata, tse 6-24, 25-29 le dissertations 30-46 li boletse tšebeliso ea P8375 liprothokholeng tsa bona tsa lipatlisiso.