M-MLV Reverse Transcriptase (Glycerol mahala)
Phetolelo ea Reverse Transcriptase e nang le lyophilizable.E ka sebelisoa ho thekenoloji ea lyophilization e tlase ha e ntse e boloka ts'ebetso e kholo ea ho fetolela le botsitso.Sehlahisoa sena ha se na li-excipients, ka kopo eketsa tsa hau ha ho hlokahala.
Likaroloana
Karolo | HC2005A-01 (10,000U) | HC2005A-02 (40,000U) |
Reverse Transcriptase (Glycerol Free) (200U/μL) | 50 μL | 200 μL |
5 × Buffer | 200 μL | 800 μL |
Kopo:
E sebetsa molemong oa karabelo ea RT-qPCR ea mohato o le mong.
Boemo ba polokelo
Boloka ho -30 ~ -15°C 'me u tsamaee ho ≤0°C.
Tlhaloso ea Yuniti
Yuniti e le 'ngoe (U) e hlalosoa e le palo ea enzyme e kenyang 1 nmol ea dTTP linthong tse sa keneng ka acid ka 10mins ho 37°C, le Poly(rA)·Oligo (dT) e le thempleite/primer.
Lintlha
Bakeng sa tšebeliso ea lipatlisiso feela.Ha e sebelisoe lits'ebetsong tsa tlhahlobo.
1.Ka kopo boloka sebaka sa liteko se hloekile;Apara liatlana le limaske tse lahloang;Sebelisa lisebelisoa tse se nang RNase joalo ka li-tubes tsa centrifuge le malebela a pipette.
2.Boloka RNA holim'a leqhoa ho qoba ho senyeha.
3.Lithempleite tsa boleng bo holimo tsa RNA li khothaletsoa ho fihlela sengoloa sa morao-rao se sebetsang hantle.