Glycerol Kinase(GK)
Tlhaloso
Protheine e kentsoeng ke lefutso lena ke ea lelapa la FGGY kinase.Protheine ena ke enzyme ea bohlokoa taolong ea ho kenngoa ha glycerol le metabolism.E susumetsa phosphorylation ea glycerol ka ATP, e fana ka ADP le glycerol-3-phosphate.Liphetoho tsa lefutso lena li amahanngoa le khaello ea glycerol kinase (GKD).Ho na le mefuta e fapaneng ea sengoloa e khouteng li-isoform tse fapaneng bakeng sa lefutso lena.
Enzyme ena e sebelisoa bakeng sa liteko tsa tlhahlobo ea tlhahlobo ea mali ho fumana triglycerides hammoho le Glycerol-3-phosphate Oxidase.
Sebopeho sa Lik'hemik'hale
Molao-motheo oa ho itšoara
Glycerol + ATP→ Glycerol -3- phosphate + ADP
Tlhaloso
Lintho tsa Tlhahlobo | Litlhaloso |
Tlhaloso | E tšoeu ho ea ho e mosehla hanyenyane phofo ea amorphous, lyophilized |
Ketsahalo | ≥15U/mg |
Bohloeki(SDS-PAGE) | ≥90% |
Solubility(10mg phofo/ml) | Hlakileng |
Catalase | ≤0.001% |
Glucose oxidase | ≤0.01% |
Uricase | ≤0.01% |
ATPase | ≤0.005% |
Hexokinase | ≤0.01% |
Lipalangoang le polokelo
Lipalangoang:E romelloa ka tlas'a -15°C
Polokelo :Boloka ho -20°C(Nako e telele), 2-8°C(Nako e khutšoanyane)
E khothaletsoa tlhahlobo hapeBophelo:Likhoeli tse 18