Glucose-6-phosphate dehydrogenase (G6PDH)
Tlhaloso
Ho haella ha Glucose 6 phosphate dehydrogenase (G6PD) ke lefutso leo lisele tse khubelu tsa mali li senyehang (hemolysis) ha 'mele o pepesehetse lijo tse itseng, lithethefatsi, tšoaetso kapa khatello ea kelello.E etsahala ha motho a le sieo kapa a e-na le maemo a tlaase a enzyme glucose-6-phosphate dehydrogenase.Enzyme ena e thusa lisele tse khubelu tsa mali hore li sebetse hantle.Matšoao nakong ea lefu la hemolytic a ka kenyelletsa moroto o lefifi, mokhathala, ho soeufala, ho otla ha pelo ka potlako, ho hema ka thata, le letlalo le mosehla (jaundice).Khaello ea G6PD e futsitsoe ka mokhoa o amanang le X-recessive mme matšoao a atile haholo ho banna (haholo-holo Maafrika a Amerika le a tsoang likarolong tse ling tsa Afrika, Asia le Mediterranean).E bakoa ke liphetoho tsa liphatsa tsa lefutso mofuteng oa G6PD.
Glucose-6-Phophate Dehydrogenase (G-6-PDH) e na le likaroloana tse peli tsa boima ba molek'hule bo lekanang.Tatelano ea amino acid ea monoma e phatlalalitsoe.G-6-PDH e sebelisitsoe litlhahlobong tsa nicotinamide adenine dinucleotide le tissue pyridine. li-nucleotides.G-6-PDH e ka boela ea sebelisoa ho tsoa ho litharollo tse entsoeng ka urea.
Glucose 6-phosphate dehydrogenase ke enzyme ea bohlokoa ea taolo mohatong oa pele oa tsela ea pentose phosphate.G-6-P-DH e oxidize glucose-6-phosphate ka pel'a NADP+ ho hlahisa 6- phosphogluconate.Polyacrylamide gel electrophoresis, mosebetsi oa staining, le liphuputso tse thibelang likokoana-hloko tsa G-6-PDH tsa anti-yeast li bontšitse hore G-6-PDH ke glycoprotein.
Sebopeho sa Lik'hemik'hale
Molao-motheo oa ho itšoara
D-Glucose-6-phosphate + NAD+→D-Glucono-δ-lactone-6-phosphate + NADH+H+
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Tlhaloso | White amorphous phofo, lyophilized |
| Ketsahalo | ≥150U/mg |
| Bohloeki(SDS-PAGE) | ≥90% |
| Solubility(10mg phofo/ml) | Hlakileng |
| NADH/NADPH oxidase | ≤0.1% |
| Glutathione reductase | ≤0.001% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| 6-Phosphogluconate dehydrogenase | ≤0.01% |
| Myokinase | ≤0.01% |
| Hexokinase | ≤0.001% |
Lipalangoang le polokelo
Lipalangoang: Ambient
Polokelo :Boloka mocheso oa 2-8 ° C
E khothaletsoa tlhahlobo hapeBophelo:2 lilemo
