D-Lactate dehydrogenase (LDH)
Tlhaloso
Enzyme e sebelisetsoa ho tlosoa ha pyruvate ha ho etsoa liqeto tse sebetsang le NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Tšebeliso
1.Alkaline phosphatase e kopantsoeng le liprotheine (li-antibodies, streptavidin joalo-joalo,) li ka khetholla ka ho khetheha limolek'hule tsa sepheo, 'me li ka sebelisoa ho ELISA, WB le ho lemoha histochemical;
2.Alkaline phosphatase e ka sebelisoa ho dephosphorize 5 '-terminal ea DNA kapa RNA ho thibela ho itšehla thajana;
3.Dephosphorylated DNA kapa RNA e ka holimo e ka ngoloa ke phosphates e ngotsoeng ka seea-le-moea (ka T4 poly-nucleotide kinase)
Sebopeho sa Lik'hemik'hale
Mokhoa oa ho arabela
D-Lactate + NAD+→ Pyruvate +NADH + H+
Tlhaloso
| Lintho tsa Tlhahlobo | Litlhaloso |
| Tlhaloso | White amorphous phofo, lyophilized |
| Ketsahalo | ≥200U/mg |
| Bohloeki(SDS-PAGE) | ≥90% |
| Solubility(10mg phofo/mL) | Hlakileng |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Lipalangoang le polokelo
Lipalangoang:AmbIent
Polokelo :Boloka ho -20°C(Nako e telele), 2-8°C (nako e khutšoanyane)
E khothaletsoa tlhahlobo hapeBophelo:2 lilemo
