Poly A Carrier RNA
Nomoro ea katse: HC4001A
Poly A, polyadenylate, ke motsoako oa 100 ~ 10000 polyadenylates, e entsoeng ka polynucleotide phosphorylase in vitro.Ka vivo, poly (a) e kenyelletsoa ho 3-terminal ea mRNA ka enzyme ho ntlafatsa botsitso ba mRNA.Tšebelisong ea nucleic acid extract, ho eketsa poly A ho lysate kapa tharollo e tlamang ho ka ntlafatsa chai ea DNA le RNA.Mochineea poly A ho ntlafatsa tlhahiso ea nucleic acid ke ka tsela e latelang:
1. Khokahano e matla le sebaka sa marang-rang sa lingoliloeng.Lingoliloeng tse ngata tsa polypropylene li na le motlakase o tsitsitseng holim'a metsi, o tla adsorb nucleic acid.Carrier RNA e ka tlatsa tsenalitlamorao tsa adsorption le ho fokotsa tahlehelo ea li-nucleic acid.
2. Etsa hore ho be le li-nuclease tse sa sebetseng: Ho na le li-nuclease tse fapa-fapaneng lisampoleng tsa likokoana-hloko letikoloho.Poly A e ka etsa inactivate trace nucleases methating ea ho ntšitsoeng kapa ea ho boloka hontlafatsa chai le botsitso ba target nucleic acids.
3. Coprecipitation: Mohatong oa tlhoekiso ea asiti ea nucleic ea pula kapa e tlamang joala, poly A e ka kopana le nucleic acid kapa ea etsa likaroloana tsa polymer hontlafatsa ho hlaphoheloa.
Maemo a polokelo
-20 ~ 8 ℃, polokelo e omileng, polokelo ea nako e telele e lokela ho behoa ho -20 ℃
Tlhaloso
| Nomoro ea CAS | 26763-19-9 |
| Ponahalo | White lyophilized phofo |
| Bohloeki | 99% |
| Boima ba Molek'hule | 700-3500 KDa |
Mokhoa oa tšebeliso
Nka tekanyo e loketseng ea phofo ea lyophilized, eketsa metsi a phekotsoeng a DEPC kapa tharollo ea letsoai la guanidine hoe qhale ka 0.1-1ug/uL, ebe o e phuthela ka tlase ebe o e boloka ho -20°C.
Lisebelisoa
1.Virus DNA / RNA extraction: ho eketsa 1-5ug Carrier RNA ho lysate ho ka ntlafatsa lihlahisoa tsa RNA / DNA, ho tsitsisa sepheo sa nucleic acid le ho qoba ho senyeha ha nucleic acid e hloekisitsoeng nakong ea polokelo.
2. Ka DNA e nyenyane / RNA e nkiloeng ka mokhoa oa membrane (<1ug), ho eketsa RNA ea mokelikeli ho 1-5ug ho thusa ho ntlafatsa chai ea nucleic acid.
3. Ka tahi e lipakeng tsa nucleic acid precipitation le mohato oa mahloriso, ho eketsoa ha 1-2ug carrier RNA ho thusa ho ntlafatsa ho hlaphoheloa ha karolo e khutšoanyane ea RNA.
4. Ka tharollo ea quantitative probe PCR reaction, ho eketsa 10-100ng carrier RNA ho tharollo ea karabelo ho thusa ho ntlafatsa kutlo le ho fokotsa C.Tboleng.
