Mohato o le mong RT-qPCR probe kit
Tlhaloso
One Step qRT-PCR Probe Kit e etselitsoe qPCR ka kotloloho e sebelisang RNA (mohlala virus RNA) joalo ka thempleite.Ka ho sebelisa li-primers tsa mofuta o itseng (GSP), mongolo o ka morao le qPCR li ka phethoa ka tšupu e le 'ngoe, ho fokotsa haholo mekhoa ea liphaephe le kotsi ea tšoaetso.E ka emisoa ho 55 ℃ ntle le ho ama ts'ebetso le kutlo ea qRT-PCR.Mohato o le Mong qRT-PCR Probe Kit e fanoe ka Master Mix.The 5 × One Step Mix e na le buffer e ntlafalitsoeng le dNTP/dUTP Mix, 'me e loketse lisebelisoa tsa ho lemoha tse khethehileng tse thehiloeng ho li-probes tse ngotsoeng ka fluorescence (mohlala, TaqMan).
Melao-motheo ea Motheo ea RT-qPCR
Tlhaloso
| Lintho tsa Tlhahlobo | Tlhaloso | Sephetho |
| (SDS LEQEPHE) Purity of Enzyme Stock(SDS LEQEPHE) | ≥95% | Feta |
| Ts'ebetso ea Endonuclease | Ha e fumanwe | Feta |
| Ts'ebetso ea Exodulease | Ha e fumanwe | Feta |
| Mosebetsi oa Rnase | Ha e fumanwe | Feta |
| Residual E.coli DNA | ＜1likopi/60 | Feta |
| Mokhoa oa ho hlahloba o sebetsang | 90%≤110% | Feta |
Likaroloana
| Likaroloana | 100rxns | 1,000rns | 5,000 rxns |
| RNase-free ddH2O | 2*1ml | 20ml | 100ml |
| 5* mohato o le mong motsoako | 600μl | 6*1ml | 30ml |
| Motsoako o le mong oa enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX reference Dye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX reference Dye 2 | 60μl | 600μl | 3*1ml |
a.Mohato o le Mong Buffer e kenyelletsa dNTP Mix le Mg2+.
b.Enzyme Mix haholo e na le reverse
transcriptase, Hot Start Taq DNA polymerase (phetoho ea antibody) le RNase inhibitor.
c.E sebelisoa ho lokisa phoso ea lithapo tsa fluorescene lipakeng tsa liliba tse fapaneng.
d.ROX: U hloka ho khetha calibration ho latela mohlala oa sesebelisoa sa tlhahlobo.
Lisebelisoa
Tlhaloso ea QPCR
Thomello le Polokelo
Ho tsamaisa ka sekepe:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -20 ℃.
Bophelo ba Shief:18khoeli
