Mohato o le mong RT-qPCR probe kit
Tlhaloso
U+ One Step RT-qPCR Probe Kit (Glycerol-free) ke glycerol-free step RT-qPCR reagent e sebelisang RNA e le template (e kang RNA virus), e loketseng nts'etsopele le moralo oa lihlahisoa tse nang le lyophilized.Sehlahisoa sena se kopanya ts'ebetso e phahameng ea mohato o le mong o inehetseng oa Reverse Transcriptase le Champagne Taq DNA Polymerase e qalang ho chesa, e nang le lisebelisoa tsa lik'hemik'hale tse omeletseng hantle, tse nang le bokhoni bo botle ba ho holisa, ho leka-lekana le ho hlaka, 'me e tsamaellana le ho omisa ho hoama ho fapaneng. dithulaganyo.Ho feta moo, tsamaiso ea dUTP/UDG e khahlanong le tšilafalo e kenngoa ka har'a reagent, e ka sebetsang mocheso oa kamore, ho felisa tšusumetso ea tšilafalo ea sehlahisoa sa amplification ho qPCR, le ho netefatsa hore liphetho li nepahetse.
Melao-motheo ea Motheo ea RT-qPCR
Tlhaloso
| Lintho tsa Tlhahlobo | Tlhaloso | Sephetho |
| (SDS LEQEPHE) Purity of Enzyme Stock(SDS LEQEPHE) | ≥95% | Feta |
| Ts'ebetso ea Endonuclease | Ha e fumanwe | Feta |
| Ts'ebetso ea Exodulease | Ha e fumanwe | Feta |
| Mosebetsi oa Rnase | Ha e fumanwe | Feta |
| E.coli e setseng ea DNA | ＜1likopi/60 | Feta |
| Mokhoa oa ho hlahloba o sebetsang | 90%≤110% | Feta |
Likaroloana
| Likaroloana | 100rxns | 1,000rns | 5,000 rxns |
| RNase-free ddH2O | 2*1ml | 20ml | 100ml |
| 5* mohato o le mong motsoako | 600μl | 6*1ml | 30ml |
| Motsoako o le mong oa enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX reference Dye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX reference Dye 2 | 60μl | 600μl | 3*1ml |
a.Mohato o le Mong Buffer e kenyelletsa dNTP Mix le Mg2+.
b.Enzyme Mix haholo e na le reverse
transcriptase, Hot Start Taq DNA polymerase (phetoho ea antibody) le RNase inhibitor.
c.E sebelisoa ho lokisa phoso ea lithapo tsa fluorescene lipakeng tsa liliba tse fapaneng.
c.ROX: U hloka ho khetha calibration ho latela mohlala oa sesebelisoa sa tlhahlobo.
Lisebelisoa
Tlhaloso ea QPCR
Thomello le Polokelo
Lipalangoang:Liphutheloana tsa leqhoa
Maemo a polokelo:Boloka ho -20 ℃.
Bophelo ba Shief:18khoeli
