Glycohemoglobin A1c ( HbA1c) Teko ea Teko
Melemo
● Ho nepahala ho phahameng
● Matla a matla a ho thibela tšitiso
● Ho ba le botsitso bo botle
Sebopeho sa Lik'hemik'hale
Lisebelisoa
Teko ea in vitro bakeng sa boikemisetso ba palo ea mahloriso a HbA1c maling a motho kaofela ho sistimi ea photometric.HbA1c ke sehlahisoa sa hemoglobin (Hb) se hlahisang karabelo e liehang le e sa khaotseng ea glycation tlas'a tsoekere e phahameng ea mali.Glucose e fetola hemoglobin ka ho khetheha masalla a eona a n-terminal valine ho etsa hemoglobin ea glycated.Tlas'a maemo a tloaelehileng a 'mele, tlhahiso ea lihlahisoa tsa karabelo ea glycosylation e seng enzymatic e lekana hantle le bongata ba li-reactants.Kaha boemo ba hemoglobin bo lula bo tsitsitse, maemo a glycosylation a ipapisitse le khatello ea tsoekere 'me a amana le bolelele ba hemoglobin le boemo ba tsoekere.Ka hona, HbA1c ke sesupo se setle sa boemo ba tsoekere maling ba bakuli likhoeling tse 2-3 tse fetileng.
Molao-motheo
Tlas'a ketso ea protease, n-terminal ea β chain ho HbA1c ea khaoloa ebe li-dipeptide tsa glycosylated lia lokolloa.Karabelo ea pele, mahloriso a Hb a ka fumanoa ka ho lekanya ho monya ha 480 nm.Karabelong ea bobeli, fructosyl peptide oxidase (FPOX) e sebetsa ho dipeptide tsa glycosylated ho hlahisa hydrogen peroxide e ka sebetsanang le li-chromogenic agents ho hlahisa monyetla ka 660nm ka pel'a peroxidase, joale mahloriso a HbA1c a ka fumanoa ka ho lekanya monyetla oa 660nm.Ho latela khatello e fumanoeng ea HbA1c le khatello ea Hb, peresente ea HbA1c(HbA1c%) e ka baloa.
E sebetsang
Hitachi 7180/7170/7060/7600 analyser ea biochemical ea othomathike、Abbot 16000、OLYMPUS AU640automatic biochemical analyzer
Li-reagents
| Likaroloana | Tsepamisa maikutlo |
| Sebaka sa 1(R1) | |
| Buffer e ntle | 100mmol/L |
| PRK | 500KU/L |
| DA-67 | 10 mmol / L |
| Li-reagents 2 (R2) | |
| Buffer e ntle | 100mmol/L |
| Fructosyl peptide oxidase | 50 KU/L |
| Reagent 3(R3) | |
| Buffer e ntle | 100mmol/L |
Lipalangoang le polokelo
Lipalangoang:Ambient
Polokelo le Botsitso:
Ho fihlela nako ea ho felloa ke nako e bontšitsoeng leiboleng, ha e bolokoa e sa buloa ho 2-8℃ 'me e sirelelitsoe leseli.Hang ha e butsoe, li-reagents li tsitsitse matsatsi a 28 ha li le sehatsetsing ho analyzer kapa sehatsetsing.
Tšilafalo ea li-reagents e tlameha ho qojoa.U se ke ua hatsetsa li-reagents.
Hang ha e qhibilihisoa, calibrator e tsitsitse matsatsi a 15 ho 2-8 ℃, taolo e tsitsitse matsatsi a 7 ho 2-8 ℃, u se ke ua qhoma.
Shelf Life:1 selemo
